Bioremediation potential of microorganisms derived from petroleum reservoirs

Abstract

Bacterial strains and metagenomic clones, both obtained from petroleum reservoirs, were evaluated for petroleum degradation abilities either individually or in pools using seawater microcosms for 21 days. Gas Chromatography-Flame Ionization Detector (GC-FID) and Gas Chromatography-Mass Spectrometry (GC-MS) analyses were carried out to evaluate crude oil degradation. the results showed that metagenomic clones 1A and 2B were able to biodegrade n-alkanes (C14 to C33) and isoprenoids (phytane and pristane), with rates ranging from 31% to 47%, respectively. the bacteria Dietzia mans CBMAI 705 and Micrococcus sp. CBMAI 636 showed higher rates reaching 99% after 21 days. the metagenomic clone pool biodegraded these compounds at rates ranging from 11% to 45%. Regarding aromatic compound biodegradation, metagenomic clones 2B and 10A were able to biodegrade up to 94% of phenanthrene and methylphenanthrenes (3-MP, 2-MP, 9-MP and 1-MP) with rates ranging from 55% to 70% after 21 days, while the bacteria Dietzia mans CBMAI 705 and Micrococcus sp. CBMAI 636 were able to biodegrade 63% and up to 99% of phenanthrene, respectively, and methylphenanthrenes (3-MP, 2-MP, 9-MP and 1-MP) with rates ranging from 23% to 99% after 21 days. in this work, isolated strains as well as metagenomic clones were capable of degrading several petroleum compounds, revealing an innovative strategy and a great potential for further biotechnological and bioremediation applications. (C) 2014 Elsevier B.V. All rights reserved.Univ Estadual Campinas, UNICAMP, Res Ctr Chem Biol & Agr CPQBA, Microbial Resources Div, BR-13148218 Paulinia, BrazilDarcy Ribeiro North Fluminense State Univ, LENEP UENF, Lab Engn & Petr Explorat, BR-27910970 Macae, RJ, BrazilUniversidade Federal de São Paulo, UNIFESP, BR-04021001 São Paulo, BrazilUniversidade Federal de São Paulo, UNIFESP, ICAQF, BR-04021001 Diadema, BrazilWeb of SciencePETROBRASFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP