Neuron-Specific Enolase Is Elevated in Asymptomatic Carriers of Leber's Hereditary Optic Neuropathy

Abstract

PURPOSE. Neuron-specific enolase (NSE) is a biomarker for neuronal stress. Leber's hereditary optic neuropathy (LHON) is a mitochondrial disease affecting retinal ganglion cells (RGC). These RGCs and their axons in the retinal nerve fiber layer (RNFL) and optic nerve head may show subclinical pathology in unaffected mutation carriers, or undergo cell death in affected patients. We hypothesize that increased levels of blood NSE may characterize LHON carriers as a biomarker of ongoing RGC stress.METHODS. Serum was obtained from 74 members of a Brazilian pedigree with LHON carrying the homoplasmic 11778/ND4 mitochondrial DNA mutation. Classified by symptoms and psychophysical metrics, 46/74 patients were unaffected mutation carriers, 14/74 were affected, and 14/74 were off-pedigree controls. Serum NSE levels were determined by ELISA specific for the gamma subunit of NSE.RESULTS. Serum NSE concentrations in carriers (27.17 +/- 39.82 mu g/L) were significantly higher than affected (5.66 +/- 4.19 mu g/L; P = 0.050) and off-pedigree controls (6.20 +/- 2.35 mu g/L; P = 0.047). of the 14/46 (30.4 %) carriers with significantly elevated NSE levels (mean 75.8 +/- 42.3 mu g/L), 9/14 (64.3%) were male. Furthermore, NSE levels were nearly three times greater in asymptomatic male carriers (40.65 +/- 51.21 mu g/L) than in asymptomatic female carriers (15.85 +/- 22.27 mu g/L; P = 0.034).CONCLUSIONS. Serum NSE levels are higher in LHON carriers compared with affected and off-pedigree individuals. A subgroup of mostly male carriers had significantly elevated serum NSE levels. Thus, male carriers are at higher risk for LHON-related neuronal stress. (Invest Ophthalmol Vis Sci. 2012;53:6389-6392) DOI:10.1167/iovs.12-9677Univ So Calif, Keck Sch Med, Dept Ophthalmol, Doheny Eye Inst, Los Angeles, CA 90033 USAVMR Inst, Huntington Beach, CA USACtr Univ Espirito Santo, Colatina, BrazilUniversidade Federal de São Paulo, Dept Ophthalmol, São Paulo, BrazilUniv Bologna, Dept Neurol Sci, Bologna, ItalyUniv Bologna, IRCCS Inst Sci Neurol, Bologna, ItalyUniversidade Federal de São Paulo, Dept Ophthalmol, São Paulo, BrazilWeb of ScienceResearch to Prevent BlindnessNEIStruggling Within Leber's FundInternational Foundation for Optic Nerve DiseasesVMR Consulting, Huntington Beach, CaliforniaNEI: EY0304