Short- and long-term physiological responses of grapevine leaves to UV-B radiation

Abstract

33 Págs., 5 Figs., 2 Tabls. Available online 6 September 2013. The final publication is available at: http://www.sciencedirect.com/science/article/pii/S016894521300188X#The present study aimed at evaluating the short- and long-term effects of UV-B radiation on leaves of grapevine Vitis vinifera (cv. Tempranillo). Grapevine fruit-bearing cuttings were exposed to two doses of supplemental biologically effective UV-B radiation (UV-BBE) under glasshouse-controlled conditions: 5.98 and 9.66 kJ m−2 day−1. The treatments were applied either for 20 days (from mid-veraison to ripeness) or 75 days (from fruit set to ripeness). A 0 kJ m−2 d−1 UV-B treatment was included as control. The main effects of UV-B were observed after the short-term exposure (20 d) to 9.66 kJ m−2 d−1. Significant decreases in net photosynthesis, stomatal conductance, sub-stomatal CO2 concentration, the actual photosystem II (PSII) efficiency, total soluble proteins and de-epoxidation state of the VAZ cycle were observed, whereas the activities of several antioxidant enzymes increased significantly. UV-B did not markedly affect dark respiration, photorespiration, the maximum potential PSII efficiency (Fv/Fm), non-photochemical quenching (NPQ), as well as the intrinsic PSII efficiency. However, after 75 d of exposure to 5.98 and 9.66 kJ m−2 d−1 UV-B most photosynthetic and biochemical variables were unaffected and there were no sign of oxidative damage in leaves. The results suggest a high long-term acclimation capacity of grapevine to high UV-B levels, associated with a high accumulation of UV-B absorbing compounds in leaves, whereas plants seemed to be tolerant to moderate doses of UV-B.This work was supported by Fundación Universitaria de Navarra, Navarra-Aquitaine Cooperation Programme (Gobierno de Navarra and Conseil Régional d’Aquitaine), Asociación de Amigos de la Universidad de Navarra (grant to J. M-L), UV4growth COST Action FA0906 and Gobierno de Aragón (A03 research group)Peer reviewe