Anthocyanins from Eugenia brasiliensis edible fruits as potential therapeutics for COPD treatment

Abstract

Nine anthocyanins (1–9) from the edible fruits of Eugenia brasiliensis were identified by HPLC-PDA and LC–MS, and seven of these are described for the first time in this Brazilian fruit. Two of the major anthocyanins, delphinidin (8) and cyanidin (9), were studied for their inhibitory activity against chemokine interleukin-8 (IL-8) production before and after cigarette smoke extract (CSE) treatment of cells. In non-treated cells the amount of IL-8 was unchanged following treatment with cyanidin and delphinidin in concentrations 0.1–10 μM. Both delphinidin (8) and cyanidin (9) decreased the production of IL-8 in treated cells, at 1 and 10 μM, respectively. Delphinidin (8) demonstrated IL-8 inhibition in the CSE treated cells in a dose-dependent manner.Support for this study was provided by NIH-NHLBI Grant 5SC1HL096016, NIH-NCCAM Grant F31AT00801, and by the Spanish Ministry of Science and Innovation postdoctoral fellowship (G.F.).Peer reviewe