Enhanced Gold Nanoparticle Based ELISA for a Breast Cancer Biomarker

Abstract

6 páginas, 6 figuras, 1 tabla.In this work, we developed an optical enzyme-linked immunosorbent assay (ELISA) immunoassay for the analysis of CA15-3 antigen, an important biomarker present in blood samples and useful for the follow-up of the medical treatment of breast cancer. Gold nanoparticles (AuNPs) were used as carriers of the signaling antibody anti-CA15-3−HRP (horseradish peroxidase) in order to achieve an amplification of the optical signal. In the range between 0 and 60 U/mL, the assay adopting AuNPs as an enhancer resulted in higher sensitivity and shorter assay time when compared to classical ELISA procedures. The application of AuNPs to the commercially available ELISA test can be useful to improve important immunoanalysis procedures where a more confident result is needed.We acknowledge funding from the “Ministerio de Ciencia e Innovación” (Madrid, Spain) for Projects MAT2008-03079/NAN and CSD2006-00012 “NANOBIOMED” (Consolider-Ingenio 2010) and CIDEM (Generalitat de Catalunya) for Project VALTEC08- 1-0007.Peer reviewe