6 pages, 3 figures, 1 table, 28 references. Available on line 30 december 2011. The authors would like to thank ABECERA for
providing the land and allowing us to use their field facilities.
Special thanks are also expressed to Joaquín Muñoz and José
Muñoz for their field work.Semiarid Mediterranean climatic conditions and intensive tillage systems accelerate soil organic matter
losses. Therefore, assessing agricultural practices that enhance storage of soil organic matter is needed.
Stratification of soil properties with soil depth, expressed as a ratio, could indicate soil quality under
different soil management. We measured soil depth stratification ratio (0–5/10–30 cm and 0–5/30–
50 cm) of soil organic C (SOC), total N, active carbon (AC), water soluble carbon (WSC), and soil enzymatic
activities [dehydrogenase activity (DHA) and b-glucosidase activity (BGA)] of a Typic Haploxerert in
southern Spain. The experimental design consisted of a split–split plot design with three replications and
soil properties evaluated at the end of 22 years. Tillage systems included conventional tillage (CT) and no
tillage (NT). Dryland, 2-year crop rotations were wheat (Triticum aestivum L.)–fallow (WF), wheat–
chickpea (Cicer arietinum L.) (WC), wheat–faba bean (Vicia faba L.) (WFb), wheat–sunflower (Helianthus
annuus L.) (WS), and continuous wheat (WW). Nitrogen fertiliser rates were 0, 50 and 150 kg N ha 1.
Stratification ratios of total N, WSC, AC, DHA, and BGA were most responsive to tillage systems; NT
greater than CT. Stratification ratios of SOC, total N, WSC, AC and BGA were most responsive to crop
rotation; WFb and WW greater than WF, WC, and WS. Stratification ratio of BGA was most responsive to
N fertiliser rate; higher rates than no fertiliser. Stratification ratios of C and N fractions and enzymatic
activities were responsive to choice of denominator used for ratio calculation. Tillage and crop rotation
had more influence than N fertiliser rate in affecting stratification ratio of C and N fractions and
enzymatic activities. Stratification ratio was relatively low (<2), perhaps due to the large shrinking and
swelling characteristics of Vertisols. Stratification ratio of BGA was greater than of all other soil
properties, suggesting that it might be a good indicator of soil quality under different soil management in
Mediterranean conditions, particularly with Vertisols. This study indicated the value of stratification
ratios to detect improvement in soil organic matter fractions and enzymatic activities (i.e. indicators of
soil quality) with adoption of improved conservation management approaches.Authors want to thank to Commission Interministerial de
Ciencia y Tecnología (CICYT) through projects (AGL2010-22050-
C03-03 and AGL2006-02127), which allowed this work to be
carried out, S. Melero thanks to CSIC for her contract through
program ‘‘JAE-Doc’’.Peer reviewe
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