Determination of the antimicrobial growth promoter moenomycin-A in chicken litter

Abstract

8 pages, 6 figures, 2 tables.-- PMID: 17996876 [PubMed].-- Printed version published on Dec 21, 2007.This study aimed to develop and optimize a method for the extraction and analysis of moenomycin antibiotics (a.k.a. flavomycin) in corn-based feed premix and in chicken litter. Moenomycin-A was isolated from chicken litter using pressurized liquid extraction followed by a solid-phase extraction (SPE) clean-up step. The highly lipophilic nature of moenomycin necessitated the use of the less hydrophobic sorbent, C4-based SPE cartridge, and a higher temperature elution solvent, methanol at 50ºC, in order to obtain satisfactory percent recoveries. After clean-up, the sample was analyzed by liquid chromatography–electrospray ionization–mass spectrometry (LC–ESI–MS). Various reversed-phase columns were examined, including C18, CN, perfluorinated C6, and a porous graphitic carbon. The set of conditions that gave the highest separation efficiency while still maintaining symmetrical peak shape was the C18 column using the H2O + 0.3% HCOOH and acetonitrile mobile phase.This material is based upon work supported by the National Science Foundation under grant no. 0233700. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the National Science Foundation. S.P. acknowledges a post-doctoral fellowship from the Spanish Ministry of Education, Culture and Science (EX2003-0687).Peer reviewe