Article thumbnail

Large-scale phenomics identifies primary and fine-tuning roles for CRKs in responses related to oxidative stress

By Gildas Bourdais, Pawel Burdiak, Adrien Guy Bernard Gauthier, Lisette Nitsch, Jarkko Tapani Salojärvi, Channabasavangowda Rayapuram, Niina Johanna Idänheimo, Kerri Alyssa Hunter, Sachie Kimura, Ebe Merilo, Aleksia Fanni Maria Vaattovaara, Krystyna Oracz, David Kaufholdt, Andres Pallon, Damar Tri Anggoro, Dawid Glow, Jennifer Lowe, Ji Zhou, Omid Safronov, Tuomas Puukko, Andreas Albert, Hans Lang, Dieter Ernst, Hannes Kollist, Mikael Johan Brosche, Jörg Durner, Jan Willem Borst, David B. Collinge, Stanislaw Karpinski, Michael F. Lyngkjaer, Silke Robatzek, Michael Alois Wrzaczek and Jaakko Sakari Kangasjärvi

Abstract

Cysteine-rich receptor-like kinases (CRKs) are transmembrane proteins characterized by the presence of two domains of unknown function 26 (DUF26) in their ectodomain. The CRKs form one of the largest groups of receptor-like protein kinases in plants, but their biological functions have so far remained largely uncharacterized. We conducted a large-scale phenotyping approach of a nearly complete crk T-DNA insertion line collection showing that CRKs control important aspects of plant development and stress adaptation in response to biotic and abiotic stimuli in a non-redundant fashion. In particular, the analysis of reactive oxygen species (ROS)-related stress responses, such as regulation of the stomatal aperture, suggests that CRKs participate in ROS/redox signalling and sensing. CRKs play general and fine-tuning roles in the regulation of stomatal closure induced by microbial and abiotic cues. Despite their great number and high similarity, large-scale phenotyping identified specific functions in diverse processes for many CRKs and indicated that CRK2 and CRK5 play predominant roles in growth regulation and stress adaptation, respectively. As a whole, the CRKs contribute to specificity in ROS signalling. Individual CRKs control distinct responses in an antagonistic fashion suggesting future potential for using CRKs in genetic approaches to improve plant performance and stress tolerance.Peer reviewe

Topics: 1184 Genetics, developmental biology, physiology, RECEPTOR-LIKE KINASE, MULTIPLE SEQUENCE ALIGNMENT, ARABIDOPSIS-THALIANA, CELL-DEATH, PROTEIN-KINASE, TRANSCRIPTIONAL REGULATION, PSEUDOMONAS-SYRINGAE, FLAGELLIN PERCEPTION, LIGHT ACCLIMATION, STOMATAL IMMUNITY
Publisher: 'Public Library of Science (PLoS)'
Year: 2015
DOI identifier: 10.1371/journal.pgen.1005373
OAI identifier: oai:helda.helsinki.fi:10138/156269
Journal:

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.

Suggested articles