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Escherichia coli bactofection using Lipofectamine

By Narayanan Kumaran, Weng Lee Choon, Radu Aurelian and Ui Hang Sim Edmund

Abstract

Successful gene delivery into mammalian cells using bactofection requires entry of the bacterial vector via cell surface integrin receptors followed by release of plasmid DNA into the cellular environment. We show, for the first time, that addition of the DNA transfection reagent Lipofectamine improves entry of invasive Escherichia coli into HeLa cells and enhances up to 2.8-fold green fluorescent protein (GFP) expression from a reporter plasmid. The addition of Lipofectamine may be applicable to other bacterial vectors to increase their DNA delivery efficiency into mammalian cells

Topics: QR Microbiology
Publisher: 'Elsevier BV'
Year: 2013
DOI identifier: 10.1016/j.ab.2013.04.010
OAI identifier: oai:ir.unimas.my:16310
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