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New strategy for the synthesis of chemically modified RNA constructs exemplified by hairpin and hammerhead ribozymes

By Afaf H. El-Sagheer and Tom Brown


The CuAAC reaction (click chemistry) has been used in conjunction with solid-phase synthesis to produce catalytically active hairpin ribozymes around 100 nucleotides in length. Cross-strand ligation through neighboring nucleobases was successful in covalently linking presynthesized RNA strands with high efficiency (transligation). In an alternative strategy, intrastrand click ligation was employed to produce a functional hammerhead ribozyme containing a novel nucleic acid backbone mimic at the catalytic site (cis-ligation). The ability to synthesize long RNA strands by a combination of solid-phase synthesis and click ligation is an important addition to RNA chemistry. It is compatible with a plethora of site-specific modifications and is applicable to the synthesis of many biologically important RNA molecules

Topics: QD, R1
Year: 2010
OAI identifier: oai:eprints.soton.ac.uk:178909
Provided by: e-Prints Soton
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