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MOESM1 of Transient co-expression with three O-glycosylation enzymes allows production of GalNAc-O-glycosylated Granulocyte-Colony Stimulating Factor in N. benthamiana

By Israel Ramírez-Alanis (5940764), Justin Renaud (611305), Silverio García-Lara (662228), Rima Menassa (145623) and Guy Cardineau (5940761)


Additional file 1: Figure S3. Impact of extraction buffer on G-CSF. A Western Blot detection of Sec-G-CSF:eYFP and Cyt-G-CSF:eYFP extracted with PBST0.1 % (gels on the left) or under reducing and denaturing extraction conditions (SDS-DTT) (gels on the right). B Band quantification of Western blot detected proteins. Samples collected at 4, 6 and 8 dpi. Four leaf discs from different leaves were collected from each biological sample. 20 μL TSP of PBST0.1 % treated sample or equivalent volume of SDS-DTT treated sample were loaded on the gel. Black arrows denote monomeric G-CSF:eYFP variant. p19: negative control. eGEHK: protein standard. Proteins were detected with GFP antibody. Band quantification of Western blot detected proteins (Graph). Columns denoted with a different letter are significantly different (p ≤ 0.05) using one-way ANOVA and followed by Tukey test. Error bars are standard deviation of the mean

Topics: Biochemistry, Cell Biology, Molecular Biology, Cancer, Infectious Diseases, Mucin-type O-glycosylation, G-CSF, Granulocyte-Colony Stimulating Factor, Nicotiana benthamiana, Pharmaceutical glycoprotein, Molecular farming
Year: 2018
DOI identifier: 10.6084/m9.figshare.7306091.v1
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Provided by: FigShare
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