Role of Chd7 in Zebrafish: A Model for CHARGE Syndrome

Abstract

<div><p>CHARGE syndrome is caused by mutations in the <em>CHD7</em> gene. Several organ systems including the retina, cranial nerves, inner ear and heart are affected in CHARGE syndrome. However, the mechanistic link between mutations in <em>CHD7</em> and many of the organ systems dysfunction remains elusive. Here, we show that Chd7 is required for the organization of the neural retina in zebrafish. We observe an abnormal expression or a complete absence of molecular markers for the retinal ganglion cells and photoreceptors, indicating that Chd7 regulates the differentiation of retinal cells and plays an essential role in retinal cell development. In addition, zebrafish with reduced Chd7 display an abnormal organization and clustering of cranial motor neurons. We also note a pronounced reduction in the facial branchiomotor neurons and the vagal motor neurons display aberrant positioning. Further, these fish exhibit a severe loss of the facial nerves. Knock-down of <em>Chd7</em> results in a curvature of the long body axis and these fish develop irregular shaped vertebrae and have a reduction in bone mineralization. <em>Chd7</em> knockdown also results in a loss of proper segment polarity illustrated by flawed <em>efnb2a</em> and <em>ttna</em> expression, which is associated with later vascular segmentation defects. These critical roles for Chd7 in retinal and vertebral development were previously unrecognized and our results provide new insights into the role of Chd7 during development and in CHARGE syndrome pathogenesis.</p> </div