Glycoform quantification of PrP^res from the Canadian BSE cases using core antibody 6H4.

Abstract

<p>The proportion of PrP^res detected as diglycosylated is approximately 60% for the C- and H-type. In comparison, the L-type had a much lower proportion of the diglycosylated PrP^res at approximately 40%. The L-type case also had a ratio of di- to monoglycosylated (D/M) PrP^res well below 2 (1.1), whereas the ratios of intensity of D/M from all other cases were above or equal to 2 when using core specific antibodies for detection. Profiles were determined by analyzing western blot reactivity using the BioRad Quantity One software (BioRad, Canada).</p><p><b><i>D</i></b>  =  Diglycosylated PrP^res; <b><i>M</i></b>  =  Monoglycosylated PrP^res; <b><i>Un</i></b>  =  Unglycosylated PrP^res; <b><i>D/M</i></b>  =  Diglycosylated PrP^res/Monoglycosylated PrP^res.</p