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Glycoform quantification of PrP<sup>res</sup> from the Canadian BSE cases using core antibody 6H4.

By Sandor Dudas (354771), Jianmin Yang (622), Catherine Graham (364675), Markus Czub (364677), Tim A. McAllister (171315), Michael B. Coulthart (364678) and Stefanie Czub (272272)


<p>The proportion of PrP<sup>res</sup> detected as diglycosylated is approximately 60% for the C- and H-type. In comparison, the L-type had a much lower proportion of the diglycosylated PrP<sup>res</sup> at approximately 40%. The L-type case also had a ratio of di- to monoglycosylated (D/M) PrP<sup>res</sup> well below 2 (1.1), whereas the ratios of intensity of D/M from all other cases were above or equal to 2 when using core specific antibodies for detection. Profiles were determined by analyzing western blot reactivity using the BioRad Quantity One software (BioRad, Canada).</p><p><b><i>D</i></b>  =  Diglycosylated PrP<sup>res</sup>; <b><i>M</i></b>  =  Monoglycosylated PrP<sup>res</sup>; <b><i>Un</i></b>  =  Unglycosylated PrP<sup>res</sup>; <b><i>D/M</i></b>  =  Diglycosylated PrP<sup>res</sup>/Monoglycosylated PrP<sup>res</sup>.</p

Topics: Medicine, Molecular Biology, Neuroscience, quantification, canadian, bse, cases, antibody
Year: 2013
DOI identifier: 10.1371/journal.pone.0010638.t003
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Provided by: FigShare
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