Co-electrospun poly(lactide-co-glycolide), gelatin, and elastin blends for tissue engineering scaffolds
Abstract
In this study, we describe composite scaffolds composed of synthetic and natural materials with physicochemical properties suitable for tissue engineering applications. Fibrous scaffolds were co-electrospun from a blend of a synthetic biodegradable polymer (poly(lactic-co-glycolic acid), PLGA, 10% solution) and two natural proteins, gelatin (denatured collagen, 8% solution) and (x-elastin (20% solution) at ratios of 3:1:2 and 2:2:2 (v/v/v). The resulting PLGA-gelatin-elastin (PGE) fibers were homogeneous in appearance with an average diameter of 380 80 mn, which was considerably smaller than fibers made under identical conditions from the starting materials (PLGA, 780 +/- 200 nm; gelatin, 447 +/- 1.23 nm; elastin, 1060 170 nm). Upon hydration, PGE fibers swelled to an average fiber diameter of 963 +/- 132 nm, but did not disintegrate. Importantly, PGE scaffolds were stable in an aqueous environment without crosslinking, and were more elastic than those made of pure elastin fibers. To investigate the cytocompatibility of PGE, we cultured H9c2 rat cardiac myoblasts and rat bone marrow stromal cells (BMSCs) on fibrous PGE scaffolds. We found that myoblasts grew equally as well or slightly better on the scaffolds than on tissue-culture plastic. Microscopic evaluation confirmed that myoblasts reached confluence on the scaffold surfaces while simultaneously growing into the scaffolds- 期刊论文
- IN-VITRO
- EXTRACELLULAR-MATRIX
- SMOOTH-MUSCLE
- COLLAGEN
- CELL
- NANOFIBER
- ADHESION
- POLYMER
- PROLIFERATION
- PROTEIN
- In vitro
- Extracellular matrix
- Smooth muscle
- Proteins
- Cell
- Adhesion
- polymer
- Proliferation
- protein
- Cement substance (Anatomy)
- Ground substance (Anatomy)
- Ground substance (Histology)
- Intercellular matrix
- Interstitial substance
- Matrix, Extracellular
- ECM
- Nonstriated muscle
- Unstriated muscle
- Involuntary muscle
- Visceral muscle
- Proteids
- Protein S
- Cofactor, Protein C
- Protein C cofactor
- Gene products
- bacteriorhodopsin
- collagen
- fibrinogen
- haemoglobin
- hemoglobin
- keratin
- myoglobin
- rhodopsin
- serum albumin
- Polypeptides
- 骨胶原
- Collogen
- 电池
- 胞腔
- 附着力
- Adherence
- Bonding
- peel strength
- tackiness
- adhesive bonding
- bonds (adhesive)
- ADHAESION
- ADHERENCE (FRANCAIS)
- HAFTUNG
- BONDING (ADHESIVE)
- BONDING (CHEMICAL)
- CHEMICAL BONDING
- COLLAGE
- KLEBEBINDUNG
- RESISTANCE A LARRACHEMENT
- SCHAELFESTIGKEIT
- adhesiveness
- bonding, chemical
- chemical bonds
- covalent bonding
- 粘合
- Adhesivity
- Sticking (adhesion)
- 聚合物
- 增殖