Plasma cortisol concentrations in farmed rainbow trout (Oncorhynchus mykiss) were used to determine the stress caused by feeding, counting, grading and harvesting. The effect of carrying out these practices with the addition of an aerator was also determined. The cortisol concentration in trout plasma was assessed using enzyme immunoassay (EIA). Pre-feeding levels were found to be 3-4 ng/ml. Feeding, counting, grading and harvesting produced significant elevations in plasma cortisol. The presence of an aerator during these practices significantly reduced this cortisol response. The plasma cortisol response during winter grading was significantly less (
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