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Use of magnetic nanoparticles to enhance biodesulfurization

By Farahnaz Ansari

Abstract

Biodesulfurization (BDS) is an alternative to hydrodesulfurization (HDS) as a method to remove sulfur from crude oil. Dibenzothiophene (DBT) was chosen as a model compound for the forms of thiophenic sulfur found in fossil fuels; up to 70% of the sulfur in petroleum is found as DBT and substituted DBTs; these compounds are however particularly recalcitrant to hydrodesulfurization, the current standard industrial method. My thesis deals with enhancing BDS through novel strains and through nanotechnology. Chapter highlights are: Chapter 2. My first aim was to isolate novel aerobic, mesophilic bacteria that can grow in mineral media at neutral pH value with DBT as the sole sulfur source. Different natural sites in Iran were sampled and I enriched, isolated and purified such bacteria. Twenty four isolates were obtained that could utilize sulfur compounds. Five of them were shown to convert DBT into HBP. After preliminary characterization, the five isolates were sent to the Durmishidze Institute of Biotechnology in Tbilisi for help with strain identification. Two isolates (F2 and F4) were identified as Pseudomonas strains, F1 was a Flavobacterium and F3 belonged to the strain of Rhodococcus. The definite identification of isolate F5 was not successful but with high probability it was a known strain. Since no new strains were apparently discovered, I did not work further in this direction. Chapter 3. In a second approach I studied the desulfurization ability of Shewanella putrefaciens strain NCIMB 8768, because in a previous investigation carried out at Cranfield University, it had been found that it reduced sulfur odour in clay. I compared its biodesulfurization activity profile with that of the widely studied Rhodococcus erythropolis strain IGTS8. However, S. putrefaciens was not as good as R. erythropolis. Chapter 4 and 5. I then turned to nanotechnology, which as a revolutionary new technological platform offers hope to solve many problems. There is currently a trend toward the increasing use of nanotechnology in industry because of its potentially revolutionary paths to innovation. I then asked how nanotechnology can contribute to enhancing the presently poor efficiency of biodesulfurization. Perhaps the most problematic difficulty is how to separate the microorganisms at the end of the desulfurization process. To make BDS more amenable, I explored the use of nanotechnology to magnetize biodesulfurizing bacteria. In other words, to render desulfurizing bacteria magnetic, I made them magnetic by decorating their outer surfaces with magnetic nanoparticles, allowing them to be separated using an external magnet. I used the best known desulfurizing bacterial strain, Rhodococcus erythropolis IGTS8. The decoration and magnetic separation worked very well. Unexpectedly, I found that the decorated cells had a 56% higher desulfurization activity compared to the nondecorated cells. I proposed that this is due to permeabilization of the bacterial membrane, facilitating the entry and exit of reactant and product respectively. Supporting evidence for enhanced permeabilization was obtained by Dr Pavel Grigoriev, Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino. In Chapter 6, to optimize attachment of the nanoparticles to the surface of the bacteria I created thin magnetic nanofilms from the nanoparticles and measured the attachment of the bacteria using a uniquely powerful noninvasive optical technique (Optical Waveguide Lightmode Spectroscopy, OWLS) to quantify the attachment and determine how the liquid medium and other factors influence the process

Topics: Biodesulfurization, Dibenzothiophene, Shewanella putrefaciens, Rhodococcus erythropolis, Magnetic Fe3O4, Nanoparticles, Black lipid membrane, Optical waveguide lightmode spectroscopy, Monolayer, Polyethylene glycol, Adsorption
Publisher: Cranfield University
Year: 2008
OAI identifier: oai:dspace.lib.cranfield.ac.uk:1826/4424
Provided by: Cranfield CERES

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Citations

  1. (2007). 1th edn.
  2. (2005). A multifunctional approach to development, fabrication, and characterization of Fe3O4 composites.
  3. (1996). A new continuous biofilm bioreactor for immobilized, oil degrading filamentous fungi. doi
  4. (1989). A new technique for the production of immobilized biocatalyst in large quantities. doi
  5. (2006). Advances in understanding bacterial outermembrane biogenesis. doi
  6. (1993). An Orphaned Child Makes Good - The Story of USDOE/PETC’s Foray into Fossil Fuel Biodesulfurization.
  7. (2003). Anaerobic biodesulfurization of thiophenes. PhD Thesis,
  8. (1998). Analysis of petroleum hydrocarbons in environmental media. Total Petroleum Hydrocarbon Criteria Working Group Series, doi
  9. (1995). Analytical Chemistry Handbook.
  10. (1992). and the interaction with proteins. doi
  11. (1997). Application of magnetic particles in immunoassays. doi
  12. (2003). Applications of magnetic nanoparticles in biomedicine. doi
  13. (1996). Aquatic Chemistry, 3 rd Ed. doi
  14. (1996). Bacterial adhesion. doi
  15. Bacterial biofilms in nature and disease, doi
  16. (2005). Bacterial cell shape. doi
  17. (1961). Bacteriological desulfurization of petroleum.
  18. (2008). Biocatalytic desulfurization (BDS) of petrodiesel fuels. doi
  19. (1999). Biocatalytic sulfur removal from fuels: applicability for producing low sulfur gasoline. doi
  20. (1999). Biodegradation of gasoline: kinetics, mass balance and fate of individual hydrocarbons. doi
  21. (2005). Biodesulfurization of dibenzothiophene and other organic sulfur compound by newly isolated Micobacterium strain ZD-M2. doi
  22. (1993). Biodesulfurization of dibenzothiophene sulfone by Arthrobacter sp. and studies with oxidized Illinois No. 6 coal. doi
  23. (2003). Biodesulfurization of fossil fuels. doi
  24. (2002). Biodesulfurization of naphthothiophene and benzothiophene through selective cleavage of carbon-sulfur bonds by Rhodococcus sp. doi
  25. (1992). Biodesulfurization of Water-Soluble Coal-Derived Material by Rhodococcus rhodochous IGTS8. doi
  26. (1997). Biodesulphurisation of dibenzothiophene in hydrophobic media by Rhodococcus sp strain IGTS8. doi
  27. (2001). Biodesulphurization of fuels : A tool for petroleum and oil industry.
  28. (2000). Bioencapsulation within synthetic polymers (part 1): solgel encapsulation of biologicals. doi
  29. (1986). Biomedical polymers: bacterial adhesion, colonization, and infection. doi
  30. (2003). Biopolymers at Interfaces, Revised and Expanded. doi
  31. (1991). Bioremediation: Waxy Crude Oils Stranded on Low-Energy Shorelines. In: doi
  32. (1982). Black lipid membrane from polymerizable lipids, doi
  33. (1998). Buffer dependence of refractive index increments of protein solutions. doi
  34. (1999). CEO Energy biosystems corporation the woodlands, texas before the senate environment and public works subcommittee on clean air, wetlands, private property and nuclear safety,
  35. (1996). Characterization of Organic Thin Films. doi
  36. (2001). characterization, and overexpression of flavin reductase involved in dibenzothiophene desulfurization by Rhodococcus erythropolis D-1. doi
  37. (2003). Chemical synthesis of magnetic nanoparticles. doi
  38. (2004). Chemically prepared magnetic nanoparticles. doi
  39. (2002). Chemostat approach for the direct evolution of biodesulfurization gain-of-function mutants. doi
  40. (1998). Concise review of mechanisms of bacterial adhesion to biomaterial surfaces. doi
  41. (1997). Conservation of plasmid-encoded dibenzothiophene desulphurisation genes in several rhodococci.
  42. (1995). Continuous process for biologic desulfurization of sulfur-bearing heterocyclic molecules. Energy BioSystem Corporation, The Woodlands,
  43. (1990). Degradation of dibenzothiophene by Brevibacterium sp. doi
  44. (2001). Deposition of highly resistive lipid bilayer on silicon - silicon dioxide electrode and incorporation of gramicidin studied by AC impedance spectroscopy. doi
  45. (2001). Desulfurization and desulfonation: applications of sulfurcontrolled gene expression in bacteria. doi
  46. (2000). Desulfurization of alkylated froms of both dibenzothiophene and benzothiophene by a single bacterial strain. doi
  47. (2005). Desulfurization of dibenzothiophene and diesel oils by bacteria. doi
  48. (2006). Desulfurization of dibenzothiophene by Bacillus subtilis recombinants carrying dszABC and dszD genes. doi
  49. (1996). Development of superparamagnetic nanoparticles for MRI: Effect of particle size, charge and surface nature on biodistribution. doi
  50. (1999). Developments in destructive and non-destructive pathways for selective desulfurizations in oil biorefining processes. doi
  51. (2001). Effect of patterns and inhomogeneities on the surface of waveguides used for optical waveguide lightmode spectroscopy applications. doi
  52. (2005). Energy efficiency improvement and cost saving opportunities for petroleum refineries. LBNL-56183 doi
  53. (2003). Enhanced desulfurization in a transposonmutation strain of Rhodococcus erythropolis.
  54. (2005). EPA proposes effective date for diesel fuel sulfur control requirements in rural Alaska. U.S. Environmental Protection Agency
  55. (2003). Essentials of Veterinary Bacteriology and Mycology, 6 th Ed,
  56. (1993). Experimental methods for investigating protein adsorption kinetics at surfaces. doi
  57. (2004). Experimental studies of bacteria–iodide adsorption interactions. doi
  58. (2001). Field assessment of nanoscale biometallic particles for groundwater treatment. doi
  59. (2003). Fingerprinting of hydrocarbon fuel contaminants: literature review. doi
  60. (2002). Handbook of Petroleum Product Analysis. doi
  61. Hazardous organic compound analysis. doi
  62. (2000). Hydrodesulfurization of hindered dibenzothiophenes: an overview. doi
  63. (1973). Identification of microbial products from dibenzothiophene and its proposed oxidation pathway. doi
  64. (2006). Immobilization of enzyme on a fibrous matrix. doi
  65. (1994). Improvement of desulfurization activity in Rhodococcus erythropolis KA2-5-1 by genetic engineering. doi
  66. (2007). Improvement of dibenzothiophene desulfurization activity by removing the gene overlap in the dsz operon. doi
  67. (2004). In vivo cancer targeting and imaging with semiconductor quantum dots. doi
  68. (2002). In vivo imaging of quantum dots encapsulated in phospholipid micelles. doi
  69. (2000). Interaction of the peptide antibiotic alamethicin with bilayer and non-bilayer forming lipids: Influence of increasing alamethicin concentration on the lipids supramolecular structures. doi
  70. (1996). Interfacial Phenomena and Bioproducts.
  71. (1972). Introduction to Magnetic Materials. doi
  72. (2001). Kinetic analysis of microbial desulfurization of model and light gas oil containing multiple alkyl dibenzothiophenes. doi
  73. (1994). Kinetic model for serum albumin adsorption: experimental verification. doi
  74. (1994). Kinetics of adhesion and spreading of animal cells. doi
  75. (1997). Kinetics of human and bovine serum albumin adsorption at silica-titania surfaces. doi
  76. (1997). Kinetics of monolayer particle deposition.
  77. (2004). Lessons from Nature. 1th edn.
  78. (2001). Long-term repeated biodesulfurization by immobilized Rhodococcus erythropolis KA2-5-1 cells. doi
  79. (2003). Low-sulfur gasoline & diesel : The key to lower vehicle emissions. International Council on Clean Transportation (ICCT)
  80. (1956). Magnetic measurements on individual microscopic ferrite particles near the single-domain. doi
  81. (2001). Magnetic nanoparticles and biosciences. Monatshefte fuer chemie, doi
  82. (2007). Magnetic nanoparticles: synthesis, protechtion, functionalization, and application. doi
  83. (2001). Magnetic properties of nanocrystalline CoFe2O4 powders prepared at room temperature: variation with crystallite size. doi
  84. (1994). Magnetic separation techniques in diagnostic microbiology.
  85. (2004). Magnetic spinel ferrite nanoparticles from microemulsions. doi
  86. (2003). Magnetite nanoparticle dispersions stabilzed with triblock copolymers. doi
  87. (1996). Measurement of adhesion and spreding kinetics of baby hamster kidney and hybridoma cells using an integrated optical method. doi
  88. (2003). Measuring distances in supported bilayers by fluorescence interference-contrast microscopy: polymer supports and SNARE proteins. doi
  89. Mechanism of the initial events in the sorption of marine bacteria to surfaces. doi
  90. (1985). Mechanisms of bacterial adhesion at solid water interfaces. doi
  91. (2000). Method and apparatus for isolation purification of biomolecules.
  92. (1999). Method of desulfurization of fossil fuel with flavoprotein.
  93. (1996). Microbial attack on sulphur-containing hydrocarbons: Implications for the biodesulphurisation of oils and coals. doi
  94. (2006). Microbial biocatalyst development to upgrade fossil fuels, doi
  95. (1999). Microbial desulfurization of a crude oil middle-distillate fraction: analysis of the extent of sulfur removal and the effect of removal on remaining sulfur.
  96. (2001). Microbial desulfurization of alkylated dibenzothiophene and alkylated benzothiophene by recombinant Rhodococcus sp. strain T09. doi
  97. (1999). Microbial desulfurization of alkylated dibenzothiophenes from a hydrodesulfurized middle distillate by Rhodococcus erythropolis I19.
  98. (1993). Microbial desulfurization of dibenzothiophene: a sulfur-specific pathway. doi
  99. (1999). Microbial desulfurization of organic sulfur compounds in petroleum. doi
  100. (1984). Microbial desulfurization of petroleum.
  101. (1980). Microbial removal of organic sulfur from crude oil and the environment: some new prospectives.
  102. (2001). Microspheres Microcapsules and Liposomes. 3, London: Citus Books, doi
  103. (2004). Nanocrystal and nanowire synthesis and dispersibility in supercritical fluids doi
  104. (2007). Nanometrology: a critical discipline for the twenty-first century. doi
  105. (1997). Nanoscale characterization of gold colloid monolayers: a comparison of four techniques, doi
  106. (2006). Necrotizing fasciitis caused by Shewanella putrefaciens in a uremic patient.
  107. (1997). Network formation of vinylester-styrene composite matrix resins.
  108. (2001). New Directions and Challenges in Electrochemistry. Bioelectrochemistry at metal/water interfaces doi
  109. (1998). Novel applications of liposomes. doi
  110. (2003). On relations between microscopic and macroscopic physicochemical properties of bacterial cell surfaces: an AFM study on Streptococcus mitis strains. doi
  111. (1995). Optical method for measurement of number and shape of attached cell in real time. doi
  112. (2002). Optical waveguide lightmode spectroscopy (OWLS) to monitor cell proliferation quantitatively. doi
  113. (2001). Ordered two-dimensional arrays of ferrite nanoparticles. doi
  114. (2006). Organic sulfur removal from catalytic diesel oil by hydrodesulfurization combined with biodesulfurization.
  115. (1999). OWLS: A versatile technique for sensing with bioarrays.
  116. (2003). Planar Lipid Bilayers (BLMs) and Their Applications. doi
  117. (1985). Plasmid-mediated degradation of dibenzothiophene by Pseudomonas species,
  118. (1996). Poly(ethylene oxide) and protein resistance principles, problems, and possibilities. doi
  119. (1998). Polyphenol control of cell spreading on glycoprotein substrates.
  120. (1994). Porosity of Pyrolyzed Sol-Gel Wave-Guides.
  121. (1997). Preparation and application of monosized magnetic particles in selective cell separation. In: Häfeli doi
  122. (2004). Preparation of poly(1-vinylimidazole)-grafted magnetic nanoparticles and their application for removal of metal ions. doi
  123. (1999). Processible nanostructured ma terials with electrical conductivity and magnetic susceptibility:preparation a n d properties of maghemite/polyaniline nanocomposite films. doi
  124. (2001). Protein adsorption kinetics under an applied electric field: an optical waveguide lighmode spectroscopy study. doi
  125. (2005). Protein complexes of the Escherichia coli envelopes. doi
  126. (1955). Pseudomonas rubescens, a new species from soluble oil emulsions.
  127. (1993). Random and cooperative sequential adsorption. doi
  128. (1992). Random sequential adsorption of anisotropic particles. II. Low coverage kinetics. doi
  129. (2003). Recent advances in petroleum microbiology. doi
  130. (1999). Refractory sulfur compounds in gas oils. Fuel Process. doi
  131. (2002). Report of the UK Advisory Group on Nanotechnology Applications,
  132. (1998). Riding the fossil fuel biodesulfurization wave. doi
  133. (1998). Ring cleavage of sulfur heterocycles: how does it happen.
  134. (2000). Selective desulfurization of dibenzothiophene by newly isolated Corynebacterium sp. doi
  135. (2005). Self-assembling nanoclusters in living systems: application for integrated photothermal nanodiagnostics and nanotherapy. doi
  136. (2001). Sonochemical synthesis and characterization of pure nanometer-sized Fe3O4 particles. doi
  137. (2003). Sonochemical synthesis, structural and magnetic properties of air-stable Fe/Co alloy nanoparticles. doi
  138. (1982). Sugar recognition is followed by energy-dependent strengthening.
  139. (1972). Sulfur compounds in crude oil. doi
  140. (1995). Sulfur-specific microbial desulfurization of sterically hindered analogs of dibenzothiophene.
  141. (1990). Sulfur-specific microbial metabolism of organic compounds. Resource Conservation Recycling, doi
  142. (2005). Superparamagnetic nanoparticles for biomedical applications: Possibilities and limitations of a new drug delivery system. doi
  143. (2006). Superparamagnetic nanoparticles for magnetic resonance imaging (MRI) diagnosis. (2006) PhD Thesis,
  144. (2006). Surface and colloid science. doi
  145. (2002). Surface modi.cation of superparamagnetic magnetite nanoparticles and their intracellular uptake. doi
  146. (2003). Synthesis and characterization of CoFe2O4 magnetic nanoparticles prepared by temperature-controlled coprecipitation method. doi
  147. (2003). Synthesis and magnetic characterization of Mn and Co spinel Ferrite-Silica nanoparticles with tunable magnetic core. doi
  148. (2004). Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications. doi
  149. (2005). Technological Advancement - A Factor
  150. (1988). The bacteria surface: general considerations towards design and function. doi
  151. (1992). The biology and genetics of the genus Rhodococcus. doi
  152. (1998). The effect of ionic strength on the adsorption of H +,Cd 2+, Pb 2+ and Cu 2+ by Bacillus subtilis and Bacillus licheniformis: a surface complexation model.
  153. (1998). The genus Rhodococcus. doi
  154. (1997). The Hofmeister series: salt and solvent effects on interfacial phenomena. doi
  155. (1996). The Iron Oxides- Structures, Properties, Reactions, Occurrence and Uses. doi
  156. (1985). The production and use of immobilized living microbial cells.
  157. (2001). Thesis,
  158. (1990). Toward sulfur-free fuels.
  159. (2004). Translocation of bacterial proteins – an overview. doi
  160. (2005). Two-stage process design for the biodesulfurization of a model diesel by a newly isolated Rhodococcus globerulus DAQ3, doi
  161. (2008). Ultrasensitive detection and molecular imaging with magnetic nanoparticles. doi
  162. (2002). Unified carrier-channel model of ion transfer across lipid bilayer membranes. doi
  163. (1993). Utilization of organosulfur compounds by axenic and mixed cultures of Rhodococcus rhodochrous IGTS8. doi
  164. What is nanotechnology? doi

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