Skip to main content
Article thumbnail
Location of Repository

Development of a modified hollow fibre assay for studying agents targeting the tumour neovasculature.

By Steven D. Shnyder, E. Jubb, J. Hasan, Patricia A. Cooper, Michael C. Bibby, G.C. Jayson and E. Pilarinou

Abstract

NoBackground: Previous studies have shown extensive vascularisation surrounding subcutaneously implanted fibres when the duration of the US National Cancer Institute (NCI) hollow fibre assay was prolonged. Materials and Methods: The feasibility of adapting the NCI assay for evaluating agents targeting the tumour vasculature was investigated in vitro and in vivo. Finally, in the optimised assay, changes in neovasculature formation around the fibres following treatment with the anti-vascular agent paclitaxel were quantified by immunohistochemistry. Results: Correlations between cell number seeded, time in culture and vascular endothelial growth factor (VEGF) secretion were seen. In vivo studies showed that transplanting single rather than 3 fibres at a site reduced inflammation, reducing the length of the fibre transplanted, as did without any significant loss in cell growth over 21 days. A statistically significant reduction in neovascularisation surrounding the fibres was seen accompanying paclitaxel treatment. Conclusion: Modifications made here to the NCI hollow fibre assay demonstrate its potential for analysing anti-tumour vasculature agents

Topics: Hollow fibre assay, Anti-vascular effects, Angiogenesis, H460 non-small cell lung carcinoma cell line, VEGF
Year: 2009
OAI identifier: oai:bradscholars.brad.ac.uk:10454/2975
Provided by: Bradford Scholars
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://hdl.handle.net/10454/29... (external link)
  • http://ar.iiarjournals.org/con... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.