Location of Repository

Decoding accuracy in eRF1 mutants and its correlation with pleiotropic quantitative traits in yeast

By Gloria H. Merritt, Wesley R. Naemi, Pierre M. Mugnier, Helen M. Webb, Mick F. Tuite and Tobias von der Haar

Abstract

Translation termination in eukaryotes typically requires the decoding of one of three stop codons UAA, UAG or UGA by the eukaryotic release factor eRF1. The molecular mechanisms that allow eRF1 to decode either A or G in the second nucleotide, but to exclude UGG as a stop codon, are currently not well understood. Several models of stop codon recognition have been developed on the basis of evidence from mutagenesis studies, as well as studies on the evolutionary sequence conservation of eRF1. We show here that point mutants of Saccharomyces cerevisiae eRF1 display significant variability in their stop codon read-through phenotypes depending on the background genotype of the strain used, and that evolutionary conservation of amino acids in eRF1 is only a poor indicator of the functional importance of individual residues in translation termination. We further show that many phenotypes associated with eRF1 mutants are quantitatively unlinked with translation termination defects, suggesting that the evolutionary history of eRF1 was shaped by a complex set of molecular functions in addition to translation termination. We reassess current models of stop-codon recognition by eRF1 in the light of these new data

Topics: QP506
Publisher: Oxford University Press
Year: 2010
OAI identifier: oai:kar.kent.ac.uk:25568

Suggested articles

Preview

Citations

  1. (2008). Distinct eRF3 requirements suggest alternate eRF1 conformations mediate peptide release during eukaryotic translation termination. doi
  2. (2006). Early nonsense: mRNA decay solves a translational problem. doi
  3. (2004). GTP hydrolysis by eRF3 facilitates stop codon decoding during eukaryotic translation termination. doi
  4. (2008). Messenger RNA surveillance systems monitoring proper translation termination. doi
  5. (2009). Molecular dissection of translation termination mechanism identiļ¬es two new critical regions in eRF1. doi
  6. (2005). Polypeptide chain termination and stop codon readthrough on eukaryotic ribosomes. doi
  7. (2007). Regulated translational bypass of stop codons in yeast. doi
  8. (2000). Terminating eukaryote translation: domain 1 of release factor eRF1 functions in stop codon recognition. doi
  9. (2003). Termination of translation: interplay of mRNA, rRNAs and release factors? doi
  10. (1995). The products of the SUP45 (eRF1) and SUP35 genes interact to mediate translation termination in Saccharomyces cerevisiae.

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.