Pemeriksaan Klinis Meacleod

vi, 412 hlm, : il. ; 19 x 26 cm

Analisis investasi pembangunan jalur ganda kereta api Tanjung Karang-Kertapati (Segmen Tanjung Karang - Prabumulih) [Tesis]

Bib. hal. 39-40xv, 38 hlm : il. ; 28 cm

Development of a new recombinant p24 ELISA system for diagnosisof bovine leukemia virus in serum and milk

AbstractBovine leukemia virus (BLV) is a retrovirus that causes enzootic bovine leucosis. Here, we designed a p24 enzyme-linkedimmunosorbent assay (ELISA) to detect antibodies specific for BLV capsid protein p24 (encoded by the gag gene) in bovineserum samples. The p24 gene was inserted into an Escherichia coli expression system, and recombinant proteins (GST-p24,p24, and His-p24) were purified. His-p24 was the most suitable antigen for using in the ELISA. The cut-off point was calculatedfrom a receiver operating characteristic curve derived from a set of 582 field samples that previously tested positiveor negative by BLV-CoCoMo-qPCR-2, which detects BLV provirus. The new p24 ELISA showed almost the same specificityand sensitivity as a commercial gp51 ELISA kit when used to test field serum samples, and allowed monitoring of p24antibodies in raw milk and whey. Comparing the results for the p24 ELISA and gp51 ELISA revealed that p24 antibodieswere detected earlier than gp51 antibodies in three out of eight calves experimentally infected with BLV, indicating improveddetection without diminishing BLV serodiagnosis. Thus, the p24 ELISA is a robust and reliable assay for detecting BLVantibodies in serum or milk, making it is a useful tool for large-scale BLV screening

Studi karakterisasi batuan beku Andesit Kabupaten Lampung Barat, Provinsi Lampung [Skripsi]

Bib. hal. 35-37xv, 34 hlm : il. ; 28 cm