Phytochemical Profile And Antioxidant Activity Of Guazuma ulmifolia Leaves Extracts Using Different Solvent Extraction

Guazuma ulmifolia is one of the common tropical plants that has long been used as a traditional medicine to reduce body weight as slimming herbs and to lower the cholesterol in the body. The leaves of G. ulmifolia contain phenolics compound such as flavonoids and tannin that contributes to its biological activities. In this research, we determined the content of total phenolics, flavonoids, tannins, antioxidant activity, and FTIR spectrum profile from four extracts of G. ulmifolia leaves. The extraction method used in this study was stepwise maceration with different solvents n-hexane, ethyl acetate, ethanol, and water. The content of total phenolics and flavonoids were found high in ethanol extract of G. ulmifolia leaves with 35.42 and 44.85 mg QE/g dry powder, respectively. The highest content of total tannins was obtained in ethyl acetate extract of G. ulmifolia leaves as 0.55%. Antioxidant activity from four extracts of G. ulmifolia leaves was measured using DPPH, CUPRAC, and reducing power method. The highest antioxidant activity for DPPH and CUPRAC method was obtained in ethyl acetate extract with the antioxidant capacity as 55.47 and 98.17 µmol trolox/g dry powder, respectively. While using reducing power assay gives the capacity 176.75 µmol trolox/g dry powder in the water extract. The pattern of FTIR spectra from four extracts of G. ulmifolia leaves gives the distinct characteristics of each spectrum profile. Principal component analysis using FTIR spectrum shows good clustering for each extract with 94% data variability (PC1= 77% and PC2 = 17%). It can be concluded that each extract of G. ulmifolia leaves gives a distinct phytochemical profile (phenolics content, flavonoids, tannins, and FTIR spectrum) that contributes to the antioxidant activity. This antioxidant activity mainly influenced by the concentration of phenolic compounds, which is known to have antioxidant properties

Self Nano-Emulsifying Drug Delivery System (SNEDDS) of Curcuma Mangga Val. Essential Oil and The Stability Study

oai:jurnal.ugm.ac.id:article/584Essential oil of Curcuma mangga Val. has been reported to have cytotoxic effect against cancer cell lines. But this oil is unstable in dispensing so that a self nano-emulsifying drug delivery system (SNEDDS) of the oil was conducted to solve the problem and improve its potency. In the study, optimization, verification, characterization, and stability test of the SNEDDS formula were carried out respectively by simplex lattice design (SLD) on Design Expert ver. 10 software, droplet size and Zeta potential determinations using particle size analyzer (PSA) instrument, as well as heating-cooling and freeze-thaw methods. The best SNEDDS formula resulted was Miglyol : Tween 80 : PEG 400 = 16.034% : 68.380% : 15.586%; with transmittance of 84.47 + 1.05%, droplet size of 15.75 nm, zeta potential of -8.54 mV, polydispersity index (PDI) of 0.188, emulsifying time of 49.67 + 1.7 seconds in distilled water, 24.33 + 4.19 seconds in artificial gastric fluid and 21.33 + 2.87 seconds in artificial intestine fluid. After a freeze-thaw test there was no change on the emulsion’s clarity, color, smell, as well as no separation, which means that the formula was stable thermodynamically. The optimum SNEDDS formula resulted has small particle size, better emulsifying time in artificial gastric and intestine fluids, as well as better thermodynamic stability, which in turn will improve the cytotoxic activity of the Curcuma mangga Val. rhizome oil toward cancer cells

Effects of Health Informations System “Dosing Gama” on Time Efficiency in Dosage Adjustment Evaluation

Indonesian Ministry of Health shows an increase in patients with impaired kidney and liver function by 10-50% since 2007. The increase is a challenge for pharmacists in conducting pharmaceutical care, which is that dose adjustment for these patients takes a long time. Dosing GAMA, an application that has been developed in 2018, is expected to overcome these obstacles. This study aims to identify the use of the Dosing GAMA application to assist pharmacists in making dose adjustments evaluation more efficient and to assess pharmacist's acceptance of the application. This study was a quasi-experimental study using post-test with control group design. Respondents were recruited by selecting pharmacists according to the inclusion criterion. The control group consisted of 26 pharmacists who made dose adjustments manually, and the intervention group consisted of 26 pharmacists who made dose adjustments using the Dosing GAMA application. Data were obtained by measuring the time required by the pharmacist to make dose adjustments. The intervention group was then asked to complete a perceived acceptance questionnaire. The study indicated that Dosing GAMA could reduce the time needed for dose adjustment evaluation (p<0.05). An average time spent by pharmacists with Dosing GAMA was shorter than that spent by those who made dose adjustments manually without application (13.81±0.78 min vs. 27.5±1.23 min). Overall, the pharmacists have high perceived acceptance of the application. The study results can be used as the basis for developing the application. Furthermore, it is expected that the Dosing GAMA can improve pharmacists’ performance in providing effective pharmaceutical care

Liquisolid Tablets Formulation of Atorvastatin Calcium Using Polyethylene Glycol 400 as Solvent and Some Carrier Materials

The dissolution of atorvastatin calcium need to be improved since included BCS Class II drugs with low solubility and high permeability, meaning that the dissolution affects the bioavailability of drugs. This research aimed to develop a formulation of a liquisolid tablet using PEG 400 as a solvent and some carrier materials in various compositions to increase the dissolution of atorvastatin calcium. Different formulations of liquisolid tablets were conducted using different quantities of carrier and coating material for adsorbing liquid solvent to produce a free-flowing and compressible powder. Avicel PH 101, Avicel PH 102, Neusilin US2 were employed as the carrier and Aerosil 200 as the coating material. A disintegrant and lubricant were then added to the formed liquisolid system and compressed into tablets by the direct compressing method. The liquisolid tablets were characterized for their tableting properties and possible drug-excipient interaction by XRD and FTIR analysis. The tableting characteristics of atorvastatin calcium liquisolid tablets were within the acceptable limits criteria. The dissolution of AA4 and NA1 liquisolid tablets was higher compared to marketed tablets. Based on the XRD and FTIR analysis, no interactions between drug and excipient. &nbsp

New curcumin analog, CCA-1.1, synergistically improves the antiproliferative effect of doxorubicin against T47D breast cancer cells

An improved compound of pentagamavunone-1 (PGV-1), chemoprevention-curcumin analog 1.1 (CCA-1.1), has been synthesized and proven to have antiproliferative effects against breast cancer cells. This study is designed to investigate the potency of CCA-1.1 alone and in combination with doxorubicin (Dox) on T47D cells in comparison with that of PGV-1. We used the MTT assay to assess cytotoxic activity. Propidium iodide (PI), annexin-V–PI, and DCFDA staining were respectively used to determine cell cycle profiles, apoptosis, and intracellular reactive oxygen species (ROS) levels. Senescent cells were identified using the SA-b-galactosidase assay. Our results revealed that CCA-1.1 possesses cytotoxic effects similar to those of PGV-1 on T47D cells. Synergistic effects during co-treatment with Dox were also observed. CCA-1.1 arrested cell cycle progression at the G2/M phase and limited sub-G1 accumulation, which is correlated with apoptosis. CCA-1.1 alone and in combination with Dox increased senescence and intracellular ROS to a similar level to those achieved by PGV-1. CCA-1.1 alone and in combination with Dox enhanced cytotoxic activity toward T47 cells compared to PGV-1. Thus, this curcumin analog may be a potential chemotherapeutic/co-chemotherapeutic candidate for estrogen receptor-positive (ER+) breast cancer therapy

An Explorative Qualitative Study on Pharmacist Active Engagement Approach in Primary Healthcare Diabetic Education

The conventional counseling method focuses on imparting knowledge meanwhile active engagement approach would enhance patient’s participation and control in the health management process. This study explored the various elements that affect the pharmacist-patient active engagement during diabetes counseling sessions in primary healthcare settings. A qualitative study using thematic analysis of semi-structured interviews was utilized. Subjects were recruited from five primary healthcare clinics in Kuala Lumpur. Ten pharmacists actively running the diabetes medication therapy adherence clinical (DMTAC) counseling and 15 patients who are currently enrolled under the DMTAC program participated in this study. A combination of computer software ATLAS.ti and hand-written methods were used in the mind mapping to assist with the development of the themes. The qualitative analysis identified themes associated with pharmacist active engagement approach include establish rapport (subthemes: introduction and greetings, ice-breaking, background check, attitude, pharmacist motivation), getting information to explore the patient problems (subthemes: giving attention, communication, the time factor, re-assess patients’ understanding), building relationship (subthemes: sharing of ideas, emotions, relationship, trust, sensitiveness, support and belief), explanation and planning (subthemes: making a decision and mutual discussion) and closing the session (subthemes: accessibility to pharmacists, DMTAC appointments). This study established areas of active engagement namely, shared decision-making, engaging communication, and motivational interview skills that should be imparted to the pharmacists to enhance the current diabetes counseling practice

The Effect of Anti Pollutant Gel from Sansevieria trifasciata on Malondialdehyde Level and Histopathology of Rats’ Liver and Lungs Induced by Cigarette, Coal, and Mosquito Smoke

Gas pollutants that accumulate in the room with restricted air circulation may cause respiratory system disorders. A pregnane glycoside compound from Sansevieria can reduce the pollutants. This study aims at producing gels from Sansevieria extract to neutralize indoor gas pollutants. Sansevieria extract is produced by the maceration process with composition 8 g simplicia and 100 ml ethanol 96%. The extract was processed into the gel with a 20% concentration. The gel was applied to rats (Rattus norvegicus) Wistar strain induced by cigarette, coal, and mosquito smoke with positive control and treatment groups. After 8 days, the Gross examination and histopathology of lungs and liver were observed quantitatively. MDA levels were measured with the TBARS method. Data were analyzed by independent sample T-test and Mann-Whitney test with p-value considered significant if <0,05. Gross examination of lungs showed a significant difference between the treatment and control group that was induced by coal smoke (p=0.031), and mosquito smoke (p=0.006), and the liver’s gross of cigarette smoke (p=0.040). Histopathology of lungs showed a significant difference in mosquito smoke (p=0.032) and no significant difference in histopathology of the liver. MDA levels showed significant difference in coal smoke (p=0.020) and mosquito smoke (p=0.000). In conclusion, anti pollutant gel reduces MDA levels and damage of the lungs induced by pollutants