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تصويب مسمي الدرجة العلمية بكالوريس الشرف في التعليم قبل المدرسي كلية التربية
No full textتصويب مسمي الدرجة العلمية بكالوريس الشرف في التعليم قبل المدرسي كلية التربي
Incidence of Malassezia pachydermatisin The Ears of Apparently Healthy Cattle
Get PDFOne hundred and fifty bovine ear swabs from crossed breeds were
investigated for the presence of Malassezia pachydermatis in the external
ear canals of apparently healthy cattle. The study included 25 calves, 100
dairy cows from different farms in Khartoum North and 25 oxen from the
El kadaro slaughter house.
Collection of the specimenswas made by sterile swabs; after cleaning
of the auricle with alcohol solution, recording amount and the nature of the
wax within the ear canal. Specimens were transported immediately after
collection to the laboratory for investigation.
Specimens were inoculated onto Sabouraud Dextrose Agar (SDA)
PH5.6; supplemented with Chloramphenicol (150 mg\L) and
Cyclohexamide (0.05 mg\L) to suppress the growth of bacteria and saprobic
fungi. Olive oil was added as a growthfactor and incubation was made at
35 C
o
for at least a week.
Two samples yielded yeast growth representing 1.3 % of the total
samples. These isolates were identified as Malassezia pachydermatis.
The isolates were identified to the species level according to the methods
described by Lodder (1974) and Geuho and Guillot, (1996).
VII
The relatively lower existence of Malassezia pachydermatisin this
study could be due to anatomical structure of the external ear canal in these
breeds also could be due to environmental temperature and humidity.
Macroscopic morphology revealed that rapid growth occurred at 35
C
o
, reaching a maximum after 72hrs on Sabouraud dextrose agar,
supplemented with Chlroamphenicol,colonies were round, convex and
smooth, white or creamy in colour then became buff to orange-beige.
Growth was good but slightly less than without antibiotics and was
enhanced when olive oil was added.
The microscopic morphology indicated that budding occurs from one
pole which gives a typical shape resembling footprint or a peanut.
MoreoverMalassezia pachydermatis does not form pseudomycelia.
Thus, judging by results in the present study it appears that the
Malassezia pachydermatismay be considered asa possible source of
infection in the auditory canal of cattl
