Temperature data measured during a heatwave experiment done September to November 2018 using reef building corals collected in Kāne'ohe Bay, O'ahu, Hawai'i

Dataset: Heatwave Experiment: Temperature HOBO LoggerTwo common reef-building corals, Montipora capitata and Pocillopora acuta, were collected from six sites in Kāne'ohe Bay, O'ahu, Hawai'i. Fragments were allowed to acclimate in experimental tanks for two weeks prior to exposure to one of the following four treatments: Ambient Temperature Ambient pCO2 (ATAC), Ambient Temperature High pCO2 (ATHC), High Temperature Ambient pCO2 (HTAC), and High Temperature High pCO2 (HTHC). The treatment period lasted for a two month period, starting on September 22nd, 2018 and lasting through November 17th, 2018. Following the stress period, coral fragments were exposed to a two-month recovery period in ambient conditions. HOBO loggers were placed in each tank and recorded temperature every ten minutes. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/884738NSF Division of Ocean Sciences (NSF OCE) OCE-175662

Seawater concentration data from an ocean acidification exposure experiment on adult Eastern oysters from Plum Island Sound in 2017

Dataset: Seawater and Extrapallial fluid ConcentrationTrace, minor, and major element data from adult Eastern oyster ocean acidification exposure experiments were conducted at the Ries Lab at the Northeastern University Marine Science Center on samples from Plum Island Sound in 2017. This dataset represents the phenotypic and molecular responses in the extrapallial fluid in the adult eastern oyster (Crassostrea virginica) exposed to experimental ocean acidification (OA) over 80 days. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/888887NSF Division of Ocean Sciences (NSF OCE) OCE-163542

Distributions and perturbations of the marine dissolved cobalt cycle in a changing ocean

Submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Marine Biogeochemistry at the Massachusetts Institute of Technology and the Woods Hole Oceanographic Institution February 2023.Cobalt is a necessary nutrient for many marine phytoplankton, but its hybrid-type nature results in small marine inventories that make it one of the scarcest bioactive trace metals in the oceans. This study examines the marine dissolved cobalt cycle in two regions: the Pacific Ocean and Antarctic coastal seas. In the North Pacific, elevated cobalt stoichiometries among phytoplankton were linked to nitrogen, iron and phosphate stress protein biomarkers at the boundaries of oceanographic provinces and upwelling zones, providing insight into the flexibility of cobalt stoichiometry. In both regions, perturbations to the marine cobalt cycle were either predicted or observed; in the equatorial Pacific, the dissolved cobalt inventory was predicted to increase by up to 28% due to the expansion of oxygen minimum zones in a warmer ocean, while in the Antarctic, melting ice shelves have the potential to shift the nutrient regime from iron limitation towards zinc and vitamin B12 limitation, resulting in higher cobalt demand and a lower dissolved cobalt inventory. When the global cobalt cycle was estimated throughout four of Earth’s systems (the lithosphere, biosphere, hydrosphere and the anthroposphere – the human environment), it was determined that the scale of the cobalt flux through the anthroposphere is only one order of magnitude lower than the inventory of the entire hydrosphere (10(9) mol Co yr-1 and 10(10) mol Co, respectively), revealing a vulnerability to anthropogenic perturbation of the marine cobalt inventory through human mining, use and disposal of cobalt if appropriate pollution abatement, disposal and recycling infrastructure is not established. In light of observed and predicted changes to cobalt biogeochemistry, this research suggests that the marine cobalt cycle is particularly vulnerable to anthropogenic perturbation from both global climate change and pollution due to its low ocean inventory and interconnection to other nutrient biogeochemical cycles.My work in the Saito Lab was supported by several National Science Foundation grants, including awards for the GEOTRACES GP15 program (OCE-1736599, OCE-1756138, OCE-1657781 and OCE-1736601), the CICLOPS expedition via the Office of Polar Programs (OPP-1643684, OPP-1644073 and OPP-1643845), and the CLIO Biogeochemical AUV project (OCE-1924554 and OCE-1658067), as well as funding from the Watson Foundation

Juvenile oyster survival from risk-addition experiment conducted on oyster reefs in the Guana Tolomato Matanzas National Estuarine Research Reserve from June to November 2012

Dataset: Results of risk-addition experiment (juvenile oyster survival)The eastern oyster (Crassostrea virginica) is a foundation species in northeast Florida estuaries, including the Guana Tolomato Matanzas National Estuarine Research Reserve (GTMNERR), where intertidal reefs are extensive. Estuarine research to assess sustainability and oyster population, plus various monitoring studies and oyster reef restoration projects have been undertaken, with an additional focus on testing theory regarding the effects of predation risk in the natural environment. As part of a study that manipulatively “pressed” risk cues onto oyster prey, a field experiment was conducted on oyster reefs in the Guana Tolomato Matanzas National Estuarine Research Reserve (Ponte Vedra Beach, Florida) from June to November 2012. Three areas within the southern areas of the GTM NERR (south of Matanzas inlet) were used in the experiment: Summer Island North (SIN), Marine Land (ML), and Pellicer Flats (PF). The SIN site occurred closest to the inlet (farthest from freshwater input), the PF site occurred farthest from the inlet and closest to freshwater input, and the ML site occurs between the inlet and the freshwater input. Oyster survival, growth, and recruitment were checked monthly. At the midpoint and conclusion of the experiment, individual oysters were also destructively sampled to quantify differences in oyster traits (shell versus tissue mass) as a function of experimental treatment and location. This submission concerns the survival of juvenile oysters. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/885259NSF Division of Ocean Sciences (NSF OCE) OCE-1736943, NSF Division of Ocean Sciences (NSF OCE) OCE-182054

Juvenile oyster condition index from risk-addition experiment conducted on oyster reefs in the Guana Tolomato Matanzas National Estuarine Research Reserve from June to November 2012

Dataset: Results of risk-addition experiment (juvenile oyster condition index)The eastern oyster (Crassostrea virginica) is a foundation species in northeast Florida estuaries, including the Guana Tolomato Matanzas National Estuarine Research Reserve (GTMNERR), where intertidal reefs are extensive. Estuarine research to assess the sustainability of oyster populations, plus various monitoring studies and oyster reef restoration projects have been undertaken, with an additional focus on testing theory regarding the effects of predation risk in the natural environment. As part of a study that manipulatively “pressed” risk cues onto oyster prey, a field experiment was conducted on oyster reefs in the Guana Tolomato Matanzas National Estuarine Research Reserve (Ponte Vedra Beach, Florida) from June to November 2012. Three sites within the southern areas of the GTM NERR (south of Matanzas inlet) were used in the experiment: Summer Island North (SIN), Marine Land (ML), and Pellicer Flats (PF). The SIN site occurred closest to the inlet (farthest from freshwater input), the PF site occurred farthest from the inlet and closest to freshwater input, while the ML site occurs between the inlet and the freshwater input. Oyster survival, growth, and recruitment were checked monthly. At the midpoint and conclusion of the experiment, individual oysters were also destructively sampled to quantify differences in oyster traits (shell versus tissue mass) as a function of experimental treatment and location. This submission concerns the traits of juvenile oysters approximately one month after the initiation of the experiment. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/885817NSF Division of Ocean Sciences (NSF OCE) OCE-1736943, NSF Division of Ocean Sciences (NSF OCE) OCE-182054

Macrofauna and larvae collected at the Auka hydrothermal vent field in Pescadero Basin in 2017

This data package provides the sampling locations and identifications for macrofauna and larvae collected at the Auka hydrothermal vent field in Pescadero Basin in 2017 and used in a study by Fleming et al. (2022). This data package contains five tables: paired tables for benthic slurps (sampling metadata and specimen counts), paired tables for plankton slurps (sampling metadata and specimen counts), and one table summarizing benthic and plankton specimens with Barcode of Life Data System (BOLD) Barcode Index Numbers (BINs). The paired data tables are partially aligned to Darwin Core event and occurrence tables for future contribution to the Ocean Biodiversity Information System (OBIS). Records for specimens in BOLD are available through the Global Biodiversity Information Facility (GBIF).Dalio Ocean Initiative and E/V Nautilus/Ocean Exploration Trus

Hydrothermal sediment temperature profiles sampled near Beggiatoa mats using Alivn's heatlow and temperature probes, deployed from R/V Atlantis cruises AT15-40, AT15-56 in the Guaymas Basin from 2008-2009

Dataset: Temperature Profiles AT15-40 and AT15-56This dataset includes temperature profiles from hydrothermal sediments sampled near Beggiatoa mats using Alivn's heatlow and temperature probes, deployed from R/V Atlantis cruises AT15-40 and AT15-56 in the Guaymas Basin from 2008-2009. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/3676NSF Division of Ocean Sciences (NSF OCE) OCE-064763

Larval fish identifications and concentration (individuals per 1000m3) estimates for all day samples.

Dataset: Larval fish identifications and concentrationsLarval fish identifications and concentration (individuals per 1000m3) estimates for all day samples. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/888753NSF Division of Ocean Sciences (NSF OCE) OCE-1737399, NSF Division of Ocean Sciences (NSF OCE) OCE-173736

Experiment Tank Conditions from a heatwave experiment done September to November 2018 using reef building corals collected in Kāne'ohe Bay, O'ahu, Hawai'i.

Dataset: Heatwave Experiment: Experiment Tank ConditionsTwo common reef-building corals, Montipora capitata and Pocillopora acuta, were collected from six sites in Kāne'ohe Bay, O'ahu, Hawai'i. Fragments were allowed to acclimate in experimental tanks for two weeks prior to exposure to one of the following four treatments: Ambient Temperature Ambient pCO2 (ATAC), Ambient Temperature High pCO2 (ATHC), High Temperature Ambient pCO2 (HTAC), and High Temperature High pCO2 (HTHC). The treatment period lasted for a two month period, starting on September 22nd, 2018 and lasting through November 17th, 2018. Following the stress period, coral fragments were exposed to a two-month recovery period in ambient conditions. This datasets includes experimental tank conditions through the acclimation, treatment, and recovery periods.  For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/884417NSF Division of Ocean Sciences (NSF OCE) OCE-175662

Single cell mass spectroscopy data collected to investigate metabolomics affected by cell-cell interactions in 2020 and 2021

Dataset: Single cell mass spec Cell-Cell interactionSingle-cell mass spectrometry (SCMS) was integrated with fluorescence microscopy to investigate metabolomics affected by cell-cell interactions in 2020 and 2021. These data were used to create a table in the publication of the results by Chen et al. (2022). For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/890877NSF Division of Ocean Sciences (NSF OCE) OCE-163463