Endangered Languages & Cultural Identity

Eye project is about finding a country or a people from a specific country that speaks a dying language and their culture is also becoming rare

Stability Analysis and Extension of a Discrete-Time Dynamical System for Scaffolded Learning

Van Geert and Steenbeek [16] proposed a coupled, delayed, discrete-time deterministic dynamical system to model scaffolded learning. We provide a detailed analysis of their model, whose global dynamics are complicated by the presence of intersecting lines of non-isolated, nonhyperbolic fixed points. We also interpret some of the trajectories in the system that have interesting dynamics in the context of the teacher-student interactions, and propose an extension to the model that simultaneously collapses the lines of fixed points to single, isolated points and is easily interpreted. These results provide the foundation for guiding the collection and integration of experimental data to apply the model to inform the teaching and learning process

Generating an in-vitro Model of MPS-IIIA “Sanfilippo Syndrome” via CRISPR/CAS9 and Exploring Potential Therapeutics in a PUI Setting

Autosomal recessive inheritance of a defective SGSH gene coding for the enzyme sulfamidase is responsible for mucopolysaccharidosis (MPS) type IIIA, characterized by the effects of heparan sulfate accumulation in lysosomes. The syndrome presents with coarse facial features, central nervous system dysfunction, behavioral difficulties, intellectual disability, sleep disturbance, hyperactivity, loss of communication skills, and loss of mobility. Failure to develop cost effective research methods and long-term treatment options leaves the average age expectancy at just 10-20 years of age. Gene editing techniques including ZFNs, TALENs, and CRISPR/Cas9 have been explored as both models and potential therapeutic interventions for mucopolysaccharidosis types I, II and III. However, an in-vitro model of MPS IIIA, the most severe type of MPS, has yet to be developed. Therefore, we present a model test an in-vitro human cell line model of MPS IIA using a K562 lymphoblast cell line, CRISPR/Cas9 gene editing, and a heparan sulfate enzyme-linked immunosorbent assay (ELISA). We hypothesized that disruption of SGSH would reduce or eliminate function of the sulfamidase enzyme, resulting in toxic heparan sulfate accumulation in the cell that could be quantified by an ELISA. Furthermore, we hypothesized Fluoxetine, an experimental drug treatment, would reduce heparan sulfate levels in the cell based on previous evidence in an MPS-IIIA murine model. Our work demonstrated the K562 lymphoblast cell line is a cost-effective and viable cell type for quantifying heparan sulfate levels. Preliminary drug treatments also suggest Fluoxetine may be capable of reducing cellular heparan sulfate levels in-vitro. Altogether, this work points to the potential of creating a sustainable cell model of MPS-IIIA, with the possibility of recapitulating specific patient mutations via the CRISPR/Cas9 editing system in the future

Policy Brief: S3592 - Increasing Medicaid Reimbursement to Match Commercial Payers for Safety Net Hospitals

A perspective on the state of Medicaid reimbursement and the impetus for chang

Children should have their voices heard and represented in child custody cases

How should children have their voices heard and represented in court? Some scholars have argued for an increase in direct testimony. Yet, with the emotional anxiety and stress that often ensues from this, it has been discouraged by many health professionals. Therefore, more indirect means of testimony are typically seen as a better option. Some examples are through an in camera testimony, being represented by a Court Appointed Special Advocate (CASA), or through a Law Guardian. I analyzed three cases in the early 2000s from three Upstate New York regions to see the effects these child advocates can have on the case decision and the children. I also created pamphlets of resources explaining why children’s voices matter in custody cases. These pamphlets were passed out across the community as well as to child advocates. This work has and can affect children and advocates across the Central NY region and beyond

Impact of Microplastics and Pesticides on Daphnia

This is a poster summarizing summer research on daphnia, in which studied the impacts of and synergistic effects of pesticides and microplastics on daphnia physiology, as well as microplastic accumulation and bio magnification

Formulation and Stability of Doxorubicin-Albumin Gel: Implication for Cancer Therapy

Albumin hydrogels have emerged as promising nanoparticle-based systems for the delivery of anticancer drugs. Gel formation is achieved by modulating factors such as pH, temperature, ionic strength, and albumin concentration, which influence the ionic crosslinking behavior of albumin. Our previous work demonstrated that divalent cations significantly enhance the formation of clear, transparent albumin gels at lower temperatures compared to monovalent cations. The sol–gel transition of thermally denatured albumin is temperature-dependent, shifting from a soluble form to a structured gel network. In this study, injectable doxorubicin (DOX)-loaded albumin hydrogels were formulated using monovalent cations (NaCl) to investigate controlled drug release and stability over 37 days. A robust, stability-indicating HPLC method was developed and validated for interday and intraday specificity to quantify doxorubicin within the gel matrix. Using this method, we successfully established the 1-month stability profile of DOX-albumin hydrogels

Boosting Breast Cancer Immunotherapy: Microparticulate Approach with Synergistic Adjuvants

Breast cancer is a common malignancy impacting women. A breast cancer vaccine has the potential to provide a non-invasive immunotherapeutic approach to cancer treatment. The proposed research explores the immune pathways activated by a spray-dried microparticulate breast cancer vaccine with dual adjuvants. This presentation provides data from T Cell Quantification and ELISA tests at varying concentrations of POLY:IC and IFA using dendritic cells (DC2.4) and T cells (EL4.IL2). This presentation was originally presented at the 2024 ASHP Midyear Meeting to other pharmacy students and professionals

The Relationship Between Health Literacy and the Success Rates of Students

In my project I am going to give a poster board presentation that explains why health literacy should be encouraged in all schools nationwide. I plan on showing the current guidelines surrounding health literacy and give solutions that benefit all students and stakeholders from part taking in a health literacy program

Social and economic disparities and equity

In todays world many people often struggle with receiving proper health car