MDP/NOD2はRAW264.7細胞におけるRANKL誘導破骨細胞分化を促進する

大阪歯科大学Osaka Dental University博士（歯学）Objective: Receptor activator of nuclear factor-κB ligand (RANKL) is intimately involved in regulating bone remodeling during osteoclast differentiation and promotion of osteoclast function. Upon binding to its receptor, RANK, RANKL activates various signaling cascades that induce osteoclast differentiation of osteoclast precursor cells into osteoclasts. In the innate immune system, host pattern recognition receptors, such as Toll-like receptors and nucleotide-binding oligomerization domain-like receptors (NLRs), detect pathogen-associated molecular patterns and elicit an immune response. The NLR, nucleotide-binding oligomerization domain 2 (NOD2), is known to bind muramyl dipeptide (MDP) and regulate inflammatory responses via nuclear factor-κB (NF-κB). The objective of this study was to investigate the effect of MDP on RANKL stimulation of osteoclast differentiation to elucidate the mechanism of bone resorption in a bacterial infection-induced inflammation model. Methods: The extent of osteoclast formation in MDP-stimulated RAW 264.7 cells was assessed using a tartrate-resistant acid phosphatase activity assay. The protein levels of intracellular signaling molecules were assessed by western blotting.Results: In RAW 264.7 cells, MDP stimulation did not affect the expression of RANK. MDP enhanced the expression of osteoclast-specific proteins, such as nuclear factor of activated T cells 1 (NFATc1) and cathepsin K, which are osteoclast differentiation markers, in RANKL-stimulated RAW 267.4 cells. Furthermore, JSH23, an NF-κB inhibitor, suppressed the expression of NFATc1 after co-stimulation with MDP and RANKL. Conclusion: MDP promoted osteoclast differentiation in RAW 267.4 cells by upregulating the activators, NF-κB and NFATc1, which are important for osteoclast differentiation, through enhancement of the RANKL signaling pathway.doctoral thesi

口腔内スキャナーによる歯列を含む口腔周囲スキャンデータを使用した3次元仮想患者表現の精確さに関するin vitro研究

大阪歯科大学Osaka Dental University博士（歯学）This in vitro study investigated the effect of skin markers on the accuracy of three-dimensional virtual patient reconstructions created using an intraoral scanner (IOS). A digital reference model (DRM) was fabricated by scanning the mannequin head with a maxillary dentition model. Perioral scans, including dentition (POSD), were acquired using two IOS systems, Medit i700 (Medit, Seoul, Republic of Republic of Korea) and Trios 4 (3shape A/S, Copenhagen, Denmark), both with and without skin markers. The nasal-glabellar region, an area relatively stable during facial movement, was used to integrate POSD data with facial data and maxillary dental models to create digital experimental models (DEMs). Accuracy was evaluated by measuring the three-dimensional positional deviations of the maxillary arch between the DRM and DEMs. The results demonstrated a significant interaction between IOS type and skin marker use. While both IOS systems showed clinically acceptable accuracy, the Medit i700 exhibited a significant improvement in accuracy when skin markers were applied, unlike the Trios 4, which showed minimal difference. This suggests that the effectiveness of skin markers is influenced by the specific scanning technology and algorithms of each IOS.doctoral thesi

（2型糖尿病発症前の歯周疾患管理が医療費に及ぼす影響）

大阪歯科大学Osaka Dental University博士（歯学）Objective: Although periodontal disease management has been shown to reduce the medical costs of diabetes mellitus, no studies have investigated management before the onset of type 2 diabetes mellitus. Therefore, this study examined the association between periodontal disease management before the onset of type 2 diabetes mellitus and medical costs. Methods: We extracted information for periodontal disease management status from Japanese medical claims and specific health check-up databases among 4010 patients with periodontal disease and type 2 diabetes mellitus aged ≥ 30 years who had continued periodontal disease management for 2 years after diabetes onset. We divided patients into two groups: those who had received periodontal disease management for 2 years before and 2 years after onset and those who had received periodontal disease management for only 2 years after the onset of type 2 diabetes mellitus. Results: The HbA1c level in patients without periodontal disease management during the year of diabetes onset improved by 0.13 % in male and 0.24% in female 2 years later, while that in the group with periodontal disease management improved by 0.49 % and 0.74%, respectively. The medical costs were significantly lower in the group with periodontal disease management 2 years later than in the group with no periodontal disease management. Conclusions: Regular periodontal disease prevention and management under professional care before type 2 diabetes mellitus onset contribute to improved health and reduced medical costs.doctoral thesi

Image-guided high-dose-rate interstitial brachytherapy with custom-made surface applicators for lower lip cancer as monotherapy. Technique, long-term clinical results, and quantitative dose-volume evaluation: A case series.

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Antimicrobial susceptibility surveillance of bacterial isolates recovered in Japan from odontogenic infections in 2018.

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Early introduction of oral feeding and its impact on full oral feeding and discharge in preterm infants: a systematic review protocol.

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Automorphisms of fine curve graphs for nonorientable surfaces.

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3次元歯根膜組織の圧縮刺激応答に与える基材の影響

大阪歯科大学Osaka Dental University博士（歯学）Abstract Purpose: The purpose of this study was to investigate the influence of substrates under the three-dimensional periodontal ligament (3D PDL) tissue on its biological functions after compressive stimulation. Methods: A 3D PDL tissue was created using a poly(L-lactic acid) (PLLA) porous scaffold impregnated with human periodontal ligament fibroblasts (hPDLFs). It was then placed on a polyetheretherketone (PEEK) substrate, which has a comparable elastic modulus to bone and was compressed (25 g/cm2) for 1, 3, and 7 days. The morphology and biological functions of the hPDLFs in the 3D PDL tissue on the PEEK substrate were evaluated and compared with those on the polystyrene (PS) substrate. Results: Compressive forces for the PLLA porous scaffold on the PEEK substrate were higher than those on the PS substrate. hPDLFs were present throughout the PLLA porous scaffold and there was no morphological change upon compressive stimulation. Increased expression of osteoclastogenic genes was observed after 3 days of compressive stimulation, while the level of these genes was increased by changing the substrate under the PDL tissue from PS to PEEK. Conclusion: The substrate placed under the PDL tissue during compressive stimulation affects the biological functions of hPDLFs.doctoral thesi

Effect of oral frailty on postoperative infection in patients undergoing abdominal visceral surgery.

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A radiomics model combining machine learning and neural networks for high-accuracy prediction of cervical lymph node metastasis on ultrasound of head and neck squamous cell carcinoma.

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