In vitro screening for salinity and drought stress tolerance in plant growth promoting bacterial strains

The present study was designed to elevate the in vitro bacterial mechanisms related to the plant growth promotion and their tolerance for sodium chloride (NaCl) and polyethylene glycol (PEG) in culture media. Total nine bacterial strains were studied for both stress tolerance under varying concentration of NaCl and PEG. Out of them, three bacterial strains namely Pseudomonas simiae AU, P. koreensis AK-1 and Carnobacterium sp. SJ-5 were found tolerate to stress and further used for biochemical characterization of ACC-deaminase, IAA and Pi-solubilization activities under both stresses. All three strains were exhibited equal amount of Pisolubilization at each stress levels. The strain P. simiae AU significantly presented the highest ACC-deaminase activity (81 nmol/mg/h and 73 nmol/mg/h) and IAA activity (41.5 Hg/mL and 39.08 Hg/mL) at 0.4M NaCl and 10% PEG stress respectively

Bio reduction of Silver ions using Centella asiatica L and its Bioactivity Investigation

In the present study to check potential effect of antimicrobial activity and leaf assisted bio reduction of silver ion using Centella asiatica has been studied. The leaves of Centella asiatica are used for antimicrobial activity and biosynthesis of silver nanoparticles. Instead the C. asiatica analysis of phytochemicals revealed the presence of alkaloids, flavanoids, tannins etc., on treatment of aqueous solution of silver nitrate with the leaf extract of c.asiatica the rapid formation of stable silver nanoparticles at high concentration is observed occur. The reduction of the metal ions and stabilization of silver nanoparticle is believed to occur by the terpenoid, flavonoid constituents of extract as well as by reducing sugar ascorbate and protein molecule in the broth. And also the plant extract were analysed antimicrobial activity of gram positive and gram negative bacteria using by agar well method supplementary 10µl, 20µl, 30µl and control which observed of result zone of inhibition. However finally conclude the promoising effect Centella asiatica leaf extract synthesis of silver nanoparticles are necessary to evaluate the positive use as a new bactericidal material

Nutrition Profile on Grape Juice – A Review

Vitis vinifera (grapes) is a well-known tropical fruit which has been used in India since 1300 AD. Grape juice is well relished by all age groups of the society. It was reported that consumption of grape juice at moderate level helps in prevention of aging related diseases. The aim of this review, a summary of the nutrient content which is available in grape juice and to obtain a further understanding of the reported beneficial health effects of the naturally occurring nutrient content in the grape juice. It has been systematically studied for various biological activities with particular reference to clinical effects. Several other potential beneficial properties in grape juice like flavonoids, polyphenols, antioxidants, anthocyanins, and resveratrol have since been ascertained. We review the potential clinical applications of these fascinating natural substances

Pesticide residue analysis in crops by LC-MS/MS method

An LC-MS-MS assay is described for fluazifop residue analysis in crops. The residues are extracted with acidified organic solvent, the esters and conjugates are hy¬drolysed with 6.01 M hydrochloric acid, then the extracts are cleaned-up by solid phase extraction using C2(EC) and Si cartridges in tandem. Quantitative analysis is performed by gradient liquid chromatography coupled to triple quad- rupole mass spectrometer using atmospheric pressure chemical ionization. All fluazifop-P-butyl, free fluazifop- P and any conjugates are quantified as fluazifop-P. The limit of quantification is 0.012-0.054 mg/kg depending on crop matrices. The clean-up method is also suitable for LC-UV analysis with a compromise in higher limit of quantification 0.052-0.22 mg/kg

Influence of Organic Bio Fertilizers on Bio Physical and Physiological Parameters in Daucua Carota L.

A field experiment was conducted at the Main Agricultural Research Station, University of Agricultural Sciences, a Dharwad during rabi season of 2006-2007 to study the effect of organics and bio fertilizers in carrot. The experiment was laid out in Randomized block design with eleven treatments and three replications. The morphological parameters viz., plant height was significantly higher due to application of RDF +Azospirillum (500g/ha) compared to other treatments. Total dry weight differed significantly among treatments at all the stages. Among treatments, significantly higher total dry weight was recorded with the application of RDF +Azospirillum (500g/ha). All growth parameters viz., Leaf area index (LAI), Absolute growth rate (AGR), Crop growth rate (CGR), Net assimilation rate (NAR) and Biomass duration (BD) were significantly higher with the application of RDF +Azospirillum (500g/ha) compared to other treatments

Organochlorine and Organophosphorus Pesticide Residues analysis by GC-MS/MS in Raw Cow Milk

Different pesticides have been used in tropical countries to control agricultural pests. Problem of the presence of pesticides in milk contributes to the serious issue of human health. The identification and quantification of Organophosphorus (Methamidophos, Acephate, Omethoate, Monocrotophous, Diazinon, Chlorpyrifos, Parathion methyl, Malathion and Ethion) and Organochlorine (Atrazine, Lindane, Dieldrin, Heptachlor, Chlordane, DDE p, p’, DDT, p, p’, Endosulphan and Imazalil) pesticides in cow milk collected and pooled from different localities of Thanjavur, Tamil Nadu was performed. Extraction was performed with 1:2 ratios of Dichloromethane and Hexane. The analysis showed that most of the pesticides were presence in the selected cow milk sample. Confirmation of pesticides was done through Scion 436 - GC Bruker with BR-5MS GC-MS/MS. Most of the Organochlorine and Organophosphorus present in milk samples. The result of the analysis showed that the cow may be exposed to pesticide rich feed which consequences the presence in milk. Hence, the intake of such milk might pose serious health hazardous to humans in this locality known tropical fruit which has been used in India since 1300 AD. Grape juice is well relished by all age groups of the society. It was reported that consumption of grape juice at moderate level helps in prevention of aging related diseases. The aim of this review, a summary of the nutrient content which is available in grape juice and to obtain a further understanding of the reported beneficial health effects of the naturally occurring nutrient content in the grape juice. It has been systematically studied for various biological activities with particular reference to clinical effects. Several other potential beneficial properties in grape juice like flavonoids, polyphenols, antioxidants, anthocyanins, and resveratrol have since been ascertained. We review the potential clinical applications of these fascinating natural substances

Studies on the Age and Growth of the Orange fin labeo, Labeo calbasu (Hamilton) from Lower Anicut, Tamil Nadu

The age determination of a carp, Labeo calbasu (Hamilton-1822) from Vadavar river Lower Anicut, Tamil Nadu has been assessed from the 110 specimens collected between April 2010 and March 2011. Using length-frequency method and scale study, it was found that Labeo calbasu attains model length of 250, 283, 385 and 442 mm at the end of zero, 1st, 2nd and 3rd years of life respectively. The relationship between the total length (TL) of the fish and scale radius (S) was found to be linear. The age estimates from scale resulted into five age groups 0 to 4 at the model length of 150, 283, 335, 395 and 442 mm. The age estimates compared the scale and length frequency methods

Assessment of Rhizobial and Phosphobacteria biofertilizer to overcome the moisture stress in the Vigna mungo

This study was undertaken to evaluate the property of Rhizobium and Phosphobacterial biofertilizer in inducing tolerance towards moisture stress. With this objective, the study was conceived by taking up pot culture studies using a mixed culture of biofertilizers (Rhizobium of pulses), phosphobacteria and silicate solubilizing bacteria. The crop plant taken for this study was Blackgram. The plant Blackgram overcomes the moisture stress by the presence of disaccharide sugar trehalose in the rhizobial cells. The other important factor was the nutrient potassium which is dissolved by silicate solubilizing culture releasing more of potassium in the soil. The increase in potassium content is directly proportional to moisture stress. Both of these enhanced the longevity of plants for few days

Role of Active Microorganisms on the Evolution of Paddy

oai:ojs2.skyfox.co:article/1The alternative sustainable agriculture is the growing interest among the scientist and farmers. The resulting bad impacts of chemical fertilizers in agriculture turn everyone’s focus on EM treated practices in food production. There are very less scientific evidences about EM treated agro products in India. Hence in the present study, an attempt is made to prove the active potentiality of the EMs in paddy growth and yield. In this study, pot culture method was carried out. The soil was obtained from Cauvery delta region. The soil quality analysis was carried out which includes pH, N, P, K, Organic carbon, Soil moisture and other essential micronutrients. Among six groups, the EM treated plants yields more as that of chemical fertilizer treated plants and the soil texture also well maintained. We suggest from our results that the EMs with vermi composting brings most efficient result in the cultivation of paddy. This combination may be used for larger agricultural practices