Percepción del sector de pequeños rumiantes de razas autóctonas sobre su resiliencia. Informe de la Tarea 2.1. Proyecto RUMIRES

El presente informe ha sido elaborado en el contexto del proyecto RUMIRES “Fortaleciendo la resiliencia de los sistemas ganaderos de pequeños rumiantes de razas locales: de la Covid-19 al cambio global” (Proyecto PID2020-120312RA-I00 financiada por MCIN/ AEI /10.13039/501100011033). En su elaboración han participado Nerea Mandaluniz Astigarraga y Joseba Lizarralde (Dpto. Producción Animal de NEIKER), Paula Gaspar García (Universidad de Extremadura), Yolanda Mena Guerrero y Juan Manuel Mancilla Leytón (Universidad de Sevilla), Barbara Soriano Martínez (CEIGRAM), Alicia Benhamou Prat y Daniel Martín Collado (Departamento de Ciencia Animal, Centro de Investigación y Tecnología Agroalimentaria de Aragón, CITA). El diseño e implementación de los grupos focales que sirven de base a este informe, así como su elaboración ha sido coordinado por Alicia Benhamou Prat y Daniel Martín Collado. El informe recoge el punto de vista de las personas participantes en los grupos focales, que fueron seleccionadas por su conocimiento y experiencia en cada uno de los casos de estudio considerados. La elaboración de este informe no habría sido posible sin su colaboración y la dedicación generosa de su tiempo, energía y experiencia, la cual agradecemos profundamente.Proyecto PID2020-120312RA-I00 financiada por MCIN/ AEI /10.13039/501100011033PublishedPROYECTO: Fortalecimiento de la resistencia de los sistemas ganaderos de pequeños rumiantes de razas locales: de COVID-19 al cambio global (RUMIRES

A critical analysis of Adaptive Box-Cox transformation for skewed distributed data management: metabolomics of Spanish and Argentinian truffles as a case study

Background Metabolic variations retrieved in metabolomic data are considered a benchmark for detecting biomatrix variability. Therefore, identifying target metabolites is crucial to keep track of any substrate modification and preserve it from any undesired alteration. Unfortunately, such a task can be negatively affected by detecting false positives, often triggered by complicated data distributions. In this work, we undertook an investigation of the metabolic profile of Spanish and Argentine truffles using a robust methodology. The issue of skewed data distributions has been effectively addressed through a normalisation preprocessing, enhancing biomarker identification and samples classification. Results A data normality-improved parametric test (ANOVA) was employed to define the target metabolites, which significantly vary between two regions of origin: Spain and Argentina. Specifically, Adaptive Box-Cox transformation was employed to improve the ANOVA test's performance so that data distributions were fitted to a Gaussian variable. Using the Bonferroni-Holm method for false discovery rate correction, we demonstrated the effectiveness of this transformation for the case under investigation. Results were compared with two non-parametric tests (Kruskall-Wallis and Permutation test), selected as a reference methodology, to provide a better understanding of non-normal distributions often encountered in metabolomic data analysis. 17 metabolites out of the 57 investigated metabolites exhibited notable variability across the two geographical regions. The validity of this methodology was supported through the discrimination of samples belonging to different groups. In this regard, both univariate and multivariate statistical models were tested through Monte Carlo simulations and yielded consistent results. Significance data analysis outcomes are sensitive to variables distributions. The present study shows an effective tool to increase data normality, thereby enhancing the statistical power for biomarker discovery and improving models’ classification performances. These results find justification from the current knowledge within the field of food sciences, enabling their application in advancing research in the truffle analysis domain.MetabolomicsFoodData preprocessingMass spectrometryGeographical originBiomarker discoveryPublishe

Grabación de la Jornada: Iniciativas Colectivas de transformación agroalimentaria

Actuación subvencionada por el Gobierno de España y el Gobierno de Aragón con cargo al Fondo de Inversiones de Terue

The efficiency of in vitro ovine embryo production using an undefined or a defined maturation medium is determined by the source of the oocyte

In vitro oocyte maturation can be influenced by oocyte source and maturation media composition. The aim of the present study was to compare the efficiency of a defined in vitro maturation medium (TCM199 supplemented with cysteamine and epidermal growth factor; Cys + EGF) with an undefined medium (TCM199 supplemented with follicle-stimulating hormone and follicular fluid; FSH + FF) for in vitro production (IVP) of ovine embryos, using oocytes obtained by laparoscopic ovum pick-up from FSH-stimulated [n=11; 158 cumulus-oocyte complexes (COCs)] and non-stimulated (n=16; 120 COCs) live ewes, as well as abattoir-derived oocytes (170 COCs). The produced blastocysts were vitrified and some of them were transferred to synchronized recipients. The best and the worst final yields of embryo IVP observed in this study were obtained using oocytes from FSH-stimulated ewes matured in FSH + FF (41.3%; 33/80) and in Cys + EGF (19.2%; 15/78) medium, respectively (p<0.01). No significant differences between both media were attained in the blastocyst development rate or in the final yield of embryo IVP using oocytes from non-stimulated ewes or abattoir-derived oocytes. The overall in vivo survival rate of the transferred vitrified blastocysts was 13.1% (8/61), without significant differences between oocyte sources or maturation media. In conclusion, under the experimental conditions of the present study, TCM199 supplemented with cysteamine and EGF is a convenient defined maturation medium for IVP of embryos from oocytes of live non-stimulated ewes or from oocytes of abattoir-derived ovaries. However, the best final yield of embryo IVP observed in this study was attained when oocytes came from FSH-stimulated donors and TCM199 was supplemented with FSH and follicular fluid.© 2010 Blackwell Verlag GmbH.This work was supported by M de Educacio´n y Ciencia (Project AGL2005-08277) and IAMZ-CIHEAM (grant for S. Hammami).Publishe

Variantes génicas asociadas con caracteres reproductivos y su utilización en el esquema de selección de ovejas de Rasa Aragonesa

La utilización de marcadores genéticos en los programas de mejora genética hace que sean más eficientes y, sobre todo, para aquellos caracteres que presentan una baja heredabilidad y una difícil y costosa medida, como son en general los caracteres reproductivos. Por otra parte, hay que tener en cuenta, que habría que validar sus efectos en poblaciones comerciales que usan tratamientos hormonales o un manejo diferente, que podrían producir interacciones y por lo tanto modificar los resultados de los genes analizados.A UPRA Grupo Pastores (Unión de Productores Grupo Raza Rasa Aragonesa) y ganaderos de Rasa Aragonesa por su implicación y participación activa en diferentes proyectos que han dado lugar al aislamiento de variantes genéticas asociadas a carácter reproductivos. Estos trabajos se han desarrollado mediante diferentes proyectos de la UE _(INTEREG-POCTEFA-EFA103/15_PIRINNOVI), Ministerio del Ciencia e Innovación (INIA RTA 2013 0041-CO-O2 y RTA2015-00090 y del Gobierno de Aragón (GCP-2019-0038-00, GCP 2022 010-00, FITE TERGENOVID, INPASS_A25_23R) así como fondos FEDERPublishe

Dietary Inclusion of Carob Pulp (Ceratonia siliqua L.) Does Not Replace the Antioxidant Effect of Vitamin E in Lambs’ Meat to Lengthen Shelf-Life

The objective of this study was to evaluate the impact of dietary carob pulp (Cp, Ceratonia siliqua L.) and vitamin E (Vit E) on the quality and shelf-life of light lamb meat stored for a maximum of 15 days under modified atmosphere packaging (MAP). Forty-eight lambs (half females and half entire males, 25.3 ± 0.3 kg of body weight and 83 ± 5.8 days old) were randomly selected and slaughtered after an experimental fattening period with a 2 × 2 factorial design: 0 vs. 20% Cp and 40 vs. 300 IU of Vit E/kg of diet for 41 days. The carcass traits, fatty acid (FA) profile, antioxidant content, colour, lipid oxidation, and microbial count in the meat were evaluated. Scarce interactions between Cp and Vit E were observed on most of the variables studied. The dietary inclusion of Cp did not affect carcass traits (p > 0.05) but produced minor changes in the content of branched FA and reduced the α-tocopherol content in the meat (p < 0.05), while no impact was observed on discolouration or lipid oxidation (p > 0.05). High Vit E supplementation increased the yellowness of caudal fat and the α-tocopherol content, which limited discolouration and lipid oxidation (p < 0.05). Furthermore, the High Vit E diet (without Cp) controlled the psychrotrophic bacterial count after 15 days of storage. At slaughter, males were heavier than females but had lower carcass dressing (p < 0.05). The lambs’ sex had minor impacts on the quality and shelf-life of meat. This study demonstrated that there is no synergistic interaction between Cp and Vit E on lamb meat quality and shelf-life. Including 20% of Cp in lamb diets is feasible without impairing meat quality. Nevertheless, supplementation with 300 IU of Vit E/kg of feed is essential to extend the shelf-life of meat stored under MAP.This research was funding by the European Union’s-H2020 research and innovation programme under the MSCA fellowship (grant No. 801586) and the Technology Transfer Operation of the Rural Development Program of Catalonia 2014–2020 (Government of Catalonia and the European Regional Development Funds, Grant code 01.02.01).tocopherolpolyphenolsmeat qualitycondensed tanninsfatty acidsPublishe

Talleres participativos para la construcción de modelos conceptuales de los sistemas de ovino de Aragón y Raza Latxa productora de queso de D.O.P Idiazabal. Informe de la Tarea 4.2. Evaluación integrada de la resiliencia usando modelos de simulación dinámica. Proyecto RUMIRES.

El presente informe se enmarca en la Tarea 4.2 del proyecto “Fortaleciendo la resiliencia de los sistemas ganaderos de pequeños rumiantes de razas locales: de la COVID-19 al cambio global (RUMIRES)”, cuyo objetivo principal es evaluar a largo plazo: i) la resiliencia de los sistemas ganaderos de pequeños rumiantes de razas locales a nivel regional, y ii) el efecto de posibles estrategias de gestión. Para lograrlo, se construirán dos modelos de simulación dinámica capaces de representar la evolución histórica de dos casos de estudio específicos: el sistema de ganadería de ovino de carne de raza Rasa Aragonesa de Aragón, y el sistema de ganadería de ovino de leche de la raza Latxa productora de quesos de D.O.P Idiazabal de Euskadi y Navarra. Este informe representa el primer paso en la construcción de estos modelos, mediante el desarrollo de un modelo conceptual para cada uno de estos casos de estudio. Estos modelos conceptuales reflejan la idea o concepción que los actores locales clave tienen sobre la estructura y funcionamiento de estos sistemas. El presente informe muestra los principales resultados obtenidos en los talleres participativos para los dos casos de estudio mencionados. El objetivo principal de estos talleres era el de mejorar los modelos conceptuales de base o modelos semilla propuestos originalmente por el equipo de investigación, a través del conocimiento experiencial de actores locales clave (i.e., expertos como ganaderos, veterinarios, técnicos e investigadores). Los talleres participativos se estructuraron en tres ejercicios de trabajo en grupo, en los cuales los participantes al taller: i) revisaron y propusieron mejoras a las variables o elementos internos del de los sistemas de estudio; ii) identificaron cuáles han sido los factores externos que han afectado a cada uno de estos sistemas, cuándo lo afectaron de manera más significativa, y cuánto lo afectaron; y iii) revisaron y propusieron mejoras sobre la representación gráfica de cómo estos factores externos se relacionan con las variables o elementos internos. Los dos casos de estudio han sido afectados por factores externos comunes (p. ej. estructuración o modernización del sector, las ayudas y reformas de la PAC, cambios en los precios de productos y de insumos), pero también por factores particulares o específicos para cada caso (p. ej. conflictos e impagos de la industria lechera en el caso de Euskadi y Navarra, o la atracción laboral hacia el sector de ganadería porcina en el caso de Aragón). La dinámica histórica de ambos casos de estudio, por su semejanza, parece indicar que algunos de los factores comunes pueden haber sido especialmente influyentes. Sin embargo, las diferencias existentes en estas dinámicas también apuntan a que la estructura interna de los dos sistemas analizados modula el impacto de los factores externos. Los participantes en el taller del caso de Aragón apuntaron a las ayudas de la PAC y sus reformas como los factores externos más influyentes en la dinámica del sistema. Los participantes en el taller del caso de Euskadi y Navarra señalaron la importancia del coste de la mano de obra, el precio del queso y las sequías (y olas de calor) Los modelos conceptuales de base, a través de estos talleres participativos, han sido enriquecidos y mejorados notablemente por los actores locales clave (expertos) de cada caso de estudio. Para el caso de Aragón, se añadieron/modificaron un total de ocho variables (dos internas, y seis externas), y 19 relaciones. Para el caso de Euskadi y Navarra, se añadieron/modificaron diez variables (cuatro internas, y seis externas), y 25 relaciones. Los modelos conceptuales mejorados serán la base para construir dos modelos dinámicos (matemáticos) que sean capaces de simular el comportamiento histórico observado de estos casos de estudio. Estos modelos cuantitativos, operativos y validados formalmente, ayudarán a evaluar diferentes estrategias de gestión bajo diferentes escenarios futuros (tanto socioeconómicos como ambientales).Proyecto PID2020-120312RA-I00 financiada por MCIN/ AEI /10.13039/501100011033PublishedPROYECTO: Fortalecimiento de la resistencia de los sistemas ganaderos de pequeños rumiantes de razas locales: de COVID-19 al cambio global (RUMIRES

Role of egg white protein gelling capacity on the processability and properties of compression-moulded films

This study aimed to assess the impact of the gelling capacity of powdered egg white protein (EWP) on the processability and properties of compressed-moulded films. Three commercial grades of EWP with varying gelling capacities and three protein-to-plasticiser ratios were selected for film preparation. The EWP structure was characterised by FTIR and XRD, and the relative abundance of proteins was estimated using LC-ESI-MS/MS. The rheological properties of the film-forming solutions (FFS), along with the mechanical, barrier, and optical properties of resulting films, were also evaluated. The EWP samples exhibited differences in secondary structure, soluble protein content, and protein profile, which were reflected in the viscosity, consistency, and elastic modulus of the FFS, all of which increased with gelling capacity. The processability and stability of the FFS were found to depend on the appropriate combination of gel strength and protein-to-plasticiser ratio. In addition to the protein-to-plasticiser ratio, the ?-turn abundance in the secondary structure of the EWP film was the parameter most strongly correlated with Young's modulus (p<0.01). However, the barrier properties of the films were significantly influenced solely by the protein-to-plasticiser ratio (p<0.01), with higher plasticiser content resulting in increased oxygen and water vapour permeability.Edible filmmolecular structurefood packagingegg white proteinglycerolcompression mouldingbarrier propertiesPublishe

Detection of SNP and validation of a SFP InDel (deletion) in inverted repeat region of the Prunus species chloroplast genome

In order to control tree size, disease, precocity and stress most Prunus varieties are cultivated as composite plants grafted onto desirable rootstocks that impart all the afore-mentioned traits. Several Prunus rootstock breeding programs have been focused on the production of interspecific hybrids. The pedigree of most of these rootstocks remains unknown due to the lack of parental information necessitating the application of DNA-based knowledge in breeding programs. The amplification and sequencing of the chloroplast inverted repeat B (IRB) region spanning 25,960. bp from P. cerasifera (myrobalan plum) Ehrh., P. amygdalus (almond) and P. persica (peach) using the ASAP method revealed a single nucleotide polymorphisms (SNP) in the rps19. rpl2 IRB region in myrobalan when compared to almond and peach. In addition, a prominent and an easily identifiable single feature polymorphism (SFP-InDel (deletion)) of 18 nucleotides was discovered in reference to the peach chloroplast genome in the ycf1 gene in the IRB region. In this work, it has been developed a highly useful polymorphic molecular marker to characterize the maternal parent in interspecific hybrids of Prunus rootstocks as a first step toward developing pedigree information. The ycf1 SFP-InDel (deletion) has been successfully used in several 3-way hybrids generated in the stone fruit rootstock breeding program for the characterization of new interspecific plant material. This SFP is expected to be highly utile in characterizing the maternal lineage of Prunus hybrids in other breeding programs. © 2014.ASAPRootstockInDelPhylogenetic analysisSFPSNPPublishe