Tracking the kinetics cellular glycolysis and glutaminolysis pathways using vibrational spectroscopy, combined with multivariate statistical and machine learning approaches for data mining

Understanding dynamic metabolic processes within living cells is crucial for gaining insights into cellular function and disease mechanisms. The kinetics of glycolysis and glutaminolysis pathways play significant roles, as alterations in their activity have been linked to various disorders, including cancer and mental health conditions such as bipolar disorder. These pathways therefore hold potential as biomarkers for disease diagnosis and therapy. However, real-time monitoring of their kinetics remains challenging due to the lack of suitable non-invasive techniques. Current gold-standard fluxomics approaches, such as mass spectrometry, are destructive to cells and thus unsuitable for time-resolved studies. In this study, we evaluate the potential of label-free vibrational spectroscopy techniques, including ATR-FTIR spectroscopy and Raman spectroscopy, as non-destructive tools for monitoring pathway kinetics and providing cellular insights into metabolic dynamics over time. Given the complexity and volume of spectral data, we also explore the application of several multivariate statistical and machine learning approaches for data mining. These include Principal Component Analysis (PCA), Linear Discriminant Analysis (LDA), and Multivariate Curve Resolution–Alternating Least Squares (MCR-ALS). Our findings highlight the potential of combining vibrational spectroscopy with advanced data analysis to enable real-time, label-free investigation of cellular metabolic pathways

NOBOCAP: UNLOCKING MDR/IVDR REGULATIONS FOR INNOVATORS IN EUROPE

The NoBoCap project (nobocap.eu) is aimed at addressing some of the challenges encountered by both SMEs and Notified Bodies across the EU. It is a multi-organizational consortium including universities, a Notified Body, and Bio-health and Innovation Hubs and Clusters. The NoBoCap project has several work packages focussed on:• Design and delivery of funded short-term courses.• Creating a dedicated NB job board.• Design and delivery of funded university accredited modules.• Design and development of e-tools to support manufacturers.• Develop a community platform to act as a voice for start-ups and SME

HyFlex Delivery for year-long 10 credit evening module

I trialled the HyFlex delivery for the LAW4021 Jurisprudence module over the full academic year. This module is a 10-credit module and is particularly relevant for those part-time LLB (TU115) students who have progressed from the undergrad BA Ord (TU112) programme, given it is a ‘core’ module. Those who may want to progress to train at the King’s Inns must take Jurisprudence, so the uptake on the module tends to be strong year-upon-year. For this reason, I thought it would be an apt choice to trial HyFlex as there is usually a critical mass in the face-to-face context, and I was certain the evening setting would ensure a good uptake online. Also, the subject matter was appropriate, as Jurisprudence focuses on questions such as the relationship between law and morality, so evening cohorts (who would tend to have a greater degree of life experience) are more vocal on the issues arising

IJRTP Volume 12(iv) Table of Contents

Special Issue: Papers Presented at Sacred Journeys 11th Global Conference - Pilgrimage: A Growing Global Phenomenon, Waijun Kaikan Conference Center, Chion-In Buddhist Temple, Kyoto, Japan, July 1-4, 202

Mobile HyFlex & Lessons Learned

My first Mobile HyFlex lecture was an insightful endeavour with 15 enthusiastic postgrad students on board, engagement was high, and their willingness to participate made for an energising experience. The high-spec hardware kit provided a solid foundation but an unstable Wi-Fi connection led to intermittent signal drops. If there is to be more widespread adoption of video camera over Wi-Fi going forward, the bandwidth requirements of the Wi-Fi network would need to be addressed and validated to ensure it can support multiple lecturers using the technology simultaneously, as this could be a bottleneck. Additionally, the camera, while impressive, seemed more suited to a conference room setting than a lecture room, thereby limiting its effectiveness. Another key consideration was class scheduling. With all classes for these two cohorts still delivered solely face-to-face, integrating HyFlex was challenging. Ideally, a fixed camera at the front of the room with wired ethernet connection could prove to be a more effective HyFlex solution than the mobile camera kit

Advancing Pharmaceutical Quality System Effectiveness

Thus, PRST recognizes that it is a timely opportunity to aggregate for all stakeholders across industry, regulatory agencies, and academia, its many years of [primarily] open access research on PQS-related topics, while continuing to drive forward with novel research. This third issue of the Journal of Applied Pharmaceutical Regulatory Science (JAPRS) marks the start of a series on PQS effectiveness, commencing with the topics of QRM and KM

GIS CPD Geospatial Skills

The GIS CPD programme is delivered to people from industry wishing to develop their Geospatial skills in GIS. For many this is an important skill. Many organisations and companies encourage and pay for their staff to attend the programme and get the CPD Students gain important skills for managing, analysing and presenting geospatial data in a GIS environment. certificate. Students come from many different industries including local authorities, semi state bodies, National mapping agency, transport consultant practices, cable companies, environmental companies. The programme has been running for almost 30 years by my former colleague Eamonn Donnelly. Up until the pandemic students attended in person and travelled from many parts of Ireland to attend. During the pandemic the course was delivered online. Based on positive feedback from students about being able to attend online the decision was taken in 2022 to offer the programme in Hyflex mode

Evaluating the GHG emissions, land use, and water use associated with contemporary dietary patterns in the Republic of Ireland

Dietary patterns are intrinsically linked to greenhouse (GHG) emissions, land use, and water use via food production systems. Analysing and comparing contemporary dietary patterns and their environmental impact is critical to identifying which should be promoted to enhance global sustainability. A cross-sectional survey of adult consumption patterns was conducted across Ireland with a representative sample size of 957 respondents. Subsequently, a farm-to-fork life cycle assessment (LCA) was employed via OpenLCA 2.0.4 to assess three primary environmental impacts (global warming, land use, and water use) across the population. Thirteen distinct dietary patterns were analysed: total population, rural, urban, self-reported (omnivore, flexitarian, pescatarian, vegetarian, and vegan), and data-derived (‘meat-focused’, ‘dairy/ovo-focused’, ‘vegetable-focused’, ‘seafood-focused’, and ‘potato-focused’). Mean calculated diet-related GHG emissions were 5.52 kgCO2eq person-1 day-1, with the ‘meat-focused’ diet representing the highest (6.62 kgCO2eq person-1 day-1) and the vegan diet being the lowest (2.0 kgCO2eq person-1 day-1). The ‘meat-focused’ diet was also associated with the highest per capita land use. The estimated mean water usage was 104 L person-1 day-1, with the ‘potato-focused’ diet using the most water and the pescatarian diet using the least. Future (2050) dietary scenarios compared to ‘business-as-usual’ patterns were projected; findings suggest substantial environmental impact reductions could be achieved if segments of the Irish population transition to vegetarian, vegan, and ‘vegetable-focused’ diets. The authors consider that employing data-derived dietary patterns (as opposed to “traditional” dietary classification) in LCA studies may yield increasingly accurate results, as dietary definitions vary significantly between individuals

Harnessing Eco-friendly Practices and Transforming Communities Through Social Entrepreneurship: Exploring the Shri Mata Vaishno Devi Shrine Mode

This study examines the influence of religious tourism on regional development, with a specific focus on eco-friendly practices and social entrepreneurship. Religious pilgrimages play a significant role in stimulating the tourism sector, hence, creating economic prospects on a global scale. Nevertheless, the extent of scholarly investigation about the impact of implementing eco-friendly practices on nearby communities, particularly within the context of religious pilgrimage destinations, remains minimal. The primary objective of this research centres on the Shri Mata Vaishno Devi shrine located in Jammu and Kashmir renowned for its widespread praise and profound cultural importance. A qualitative methodology was employed to conduct face-to-face interviews with key players involved in religious tourism services and entrepreneurship, including Yatris (pilgrims) and the local community. Thematic analysis was utilised in this study to identify eco-friendly activities that are being adopted at a shrine, which produced the following themes - environmental conservation, green transportation, healthy living, green foods, green infrastructure, and green packaging. These practices have not only made significant contributions to the preservation of the environment, but they have also facilitated the advancement of local communities, the creation of employment possibilities, and the growth of social entrepreneurship. The study suggests that the adoption of eco-friendly practices has the potential to stimulate regional development and yield economic advantages. The results of this study provide a foundation for the development of sustainable tourism strategies and the promotion of community engagement

ICMS3 Explainer Infographic

International standardisation is important for consistency in climate and financial governance. EU and Irish policy is moving to require Life Cycle Costing (LCC) and Life Cycle Carbon Emissions (LCCE) reporting