Evaluation of the DNDC Model to Estimate Soil Parameters, Crop Yield and Nitrous Oxide Emissions for Alternative Long-Term Multi-Cropping Systems in the North China Plain

Funding Information: Funding: National Natural Science Foundation of China (41830751), Hainan University Startup Fund (KYQD(ZR)-20098), the N Circle–a BBSRC-Newton Funded project (BB/N013484/1) and EU Horizon 2020 Programme (Super-G). We thank Bing Gao for providing measurement datasets and Xinping Chen for managing the field trial.Peer reviewedPublisher PD

Nitrogen Surplus Benchmarks for Controlling N Pollution in the Main Cropping Systems of China

This study was financially supported by National Key Research and Development Project of China (2017YFD0200105), China−UK PhD Placement Programme funded by CSC (201603780082), and contributes to “N-Circle” and “CINAg” projects funded by the Newton Fund via UK BBSRC/NERC (grants BB/N013484/1 and BB/N013468/1, respectively).Peer reviewedPostprintPostprin

Calibration and validation of the DNDC model to estimate nitrous oxide emissions and crop productivity for a summer maize-winter wheat double cropping system in Hebei, China

We thank Dr. Min Liu for providing the field measurement data and Prof. Xinping Chen for providing the data of crop yields. We acknowledge the financial supported from the N Circle - a BBSRC-Newton Funded project (BB/N013484/1), the National Natural Science Foundation of China (41830751) and EU Horizon 2020 programme (SuperG).Peer reviewedPostprin

Mitigating nitrous oxide emissions from agricultural soils by precision management

Nitrous oxide (N2O) emissions make up a significant part of agricultural greenhouse gas emissions. There is an urgent need to identify new approaches to the mitigation of these emissions with emerging technology. In this short review four approaches to precision managements of agricultural systems are described based on examples of work being undertaken in the UK and New Zealand. They offer the opportunity for N2O mitigation without any reduction in productivity. These approaches depend upon new sensor technology, modeling and spatial information with which to make management decisions and interventions that can both improve agricultural productivity and environmental protection

Chinese cropping systems are a net source of greenhouse gases despite soil carbon sequestration

This work was funded by National Basic Research Program of China (2014CB953800), Young Talents Projects of the Institute of Urban Environment, Chinese Academy of Sciences (IUEMS201402), National Natural Science Foundation of China (41471190, 41301237, 71704171), China Postdoctoral Science Foundation (2014T70144) and Discovery Early Career Researcher Award of the Australian Research Council (DE170100423). The work contributes to the UK-China Virtual Joint Centres on Nitrogen “N-Circle” and “CINAg” funded by the Newton Fund via UK BBSRC/NERC (grants BB/N013484/1 and BB/N013468/1, respectively).Peer reviewedPostprintPostprin

DNA Methylation and Gene Expression of Matrix Metalloproteinase 9 Gene in Deficit and Non-deficit Schizophrenia

The biological pathology of deficit schizophrenia (DS) remains unclear. Matrix metalloproteinase 9 (MMP9) might be associated with neural plasticity and glutamate regulation, involved in schizophrenia pathogenesis. This study explores gene expression and DNA methylation of MMP9 in peripheral blood mononuclear cells (PBMCs) and their relationship with clinical symptoms in DS and non-deficit schizophrenia (NDS). Pyrosequencing was used to determine DNA methylation at CpG sites in exon 4 and exon 5 of MMP9 in 51 DS patients, 53 NDS patients and 50 healthy subjects (HC). RT-qPCR was used to detect MMP9 expression. Clinical symptoms were assessed by BPRS, SANS and SAPS scales. MMP9 expression in PBMCs was significantly higher in DS than NDS and HC subjects. Compared to NDS patients, DS patients had significantly lower DNA methylation at individual CpG sites in exon 4 and exon 5 of MMP9. Correlation analysis showed that DNA methylation in exon 4 was negatively correlated with gene expression in DS group. Positive correlation was found between MMP9 expression and negative symptoms in total schizophrenic patients. The social amotivation factor of SANS and negative syndrome of BPRS was negatively correlated with DNA methylation of CpG5-1 in DS patients but not in NDS patients. DS patients showed a specific abnormality of peripheral MMP9 expression and DNA methylation, indicating a pathological mechanism underlying DS as a specific subgroup of schizophrenia

Toward a generic analytical framework for sustainable nitrogen management: application for China

Managing reactive nitrogen (Nr) to achieve a sustainable balance between production of food, feed and fiber, and environmental protection is a grand challenge in the context of an increasingly affluent society. Here, we propose a novel framework for national nitrogen (N) assessments enabling a more consistent comparison of the uses, losses and impacts of Nr between countries, and improvement of Nr management for sustainable development at national and regional scales. This framework includes four key components: national scale N budgets, validation of N fluxes, cost-benefit analysis and Nr management strategies. We identify four critical factors for Nr management to achieve the sustainable development goals: N use efficiency (NUE), Nr recycling ratio (e.g., ratio of livestock excretion applied to cropland), human dietary patterns and food waste ratio. This framework was partly adopted from the European Nitrogen Assessment and now is successfully applied to China, where it contributed to trigger policy interventions toward improvements for future sustainable use of Nr. We demonstrate how other countries can also benefit from the application our framework, in order to include sustainable Nr management under future challenges of growing population, hence contributing to the achievement of some key sustainable development goals (SDGs)

Mycorrhiza-mediated recruitment of complete denitrifying Pseudomonas reduces N₂O emissions from soil

Background Arbuscular mycorrhizal fungi (AMF) are key soil organisms and their extensive hyphae create a unique hyphosphere associated with microbes actively involved in N cycling. However, the underlying mechanisms how AMF and hyphae-associated microbes may cooperate to influence N2O emissions from “hot spot” residue patches remain unclear. Here we explored the key microbes in the hyphosphere involved in N2O production and consumption using amplicon and shotgun metagenomic sequencing. Chemotaxis, growth and N2O emissions of isolated N2O-reducing bacteria in response to hyphal exudates were tested using in vitro cultures and inoculation experiments. Results AMF hyphae reduced denitrification-derived N2O emission (max. 63%) in C- and N-rich residue patches. AMF consistently enhanced the abundance and expression of clade I nosZ gene, and inconsistently increased that of nirS and nirK genes. The reduction of N2O emissions in the hyphosphere was linked to N2O-reducing Pseudomonas specifically enriched by AMF, concurring with the increase in the relative abundance of the key genes involved in bacterial citrate cycle. Phenotypic characterization of the isolated complete denitrifying P. fluorescens strain JL1 (possessing clade I nosZ) indicated that the decline of net N2O emission was a result of upregulated nosZ expression in P. fluorescens following hyphal exudation (e.g. carboxylates). These findings were further validated by re-inoculating sterilized residue patches with P. fluorescens and by an 11-year-long field experiment showing significant positive correlation between hyphal length density with the abundance of clade I nosZ gene. Conclusions The cooperation between AMF and the N2O-reducing Pseudomonas residing on hyphae significantly reduce N2O emissions in the microsites. Carboxylates exuded by hyphae act as attractants in recruiting P. fluorescens and also as stimulants triggering nosZ gene expression. Our discovery indicates that reinforcing synergies between AMF and hyphosphere microbiome may provide unexplored opportunities to stimulate N2O consumption in nutrient-enriched microsites, and consequently reduce N2O emissions from soils. This knowledge opens novel avenues to exploit cross-kingdom microbial interactions for sustainable agriculture and for climate change mitigation