柱层析和MALDI-TOF质谱技术筛选食道癌血清标志多肽

目的探索建立柱层析和基质辅助激光解吸电离飞行时间(MALD I-TOF)质谱技术筛选食道癌血清标志多肽的方法。方法选用Sephadex G-100葡聚糖作为分离介质,自制小型分离层析柱,分别采集30名健康人、30例食道良性疾病患者和30例食道癌患者血清进行多肽分离,结合MALD I-TOF质谱仪鉴定。结果通过多肽质荷比(m/z)比对技术,筛选出4种诊断食道癌疾病的潜在血清标志多肽,m/z分别为7 651.76、8 091.34、9 264.98、11 784.79。结论柱层析和质谱技术筛选食道癌血清标志多肽所建立的分析技术不仅快速简单,而且同样适合于筛选其他人类重大疾病的血清标志多肽或酶蛋白

Third-party audit and optimization design of high-rise structure beyond code-specification of one hotel tower

某酒店塔楼是一栋180m(含3层避难层)的超限高层建筑,采用框架-核心筒结构,在34层位置有结构转换。在对结构设计进行结构体系、结构布置、构件设计和施工图设计审核的基础上,从主体框架-核心筒结构体系的构件布置和构件设计、楼盖体系的布置和构件设计、荷载取值、计算配筋和施工图配筋等方面进行了结构设计优化。采用盈建科、SATWE、MIDAS Building等多种计算软件进行了小震下结构整体内力位移分析;采用EPDA&PUSH对结构在大震下的整体内力位移进行了分析计算。立足规范和结构性能要求,对结构设计提出了修改意见和建议。One hotel tower is a 180 meters high( including 3 refuge storey) high-rise building beyond code-specification,adopts the structure system of reinforced concrete frame-core wall and structure transformation at 34 th storey. Based on the structure system,structural layout,component design and construction design review of the structural design,the structural design optimization is applied to aspects on component layout and component design of the main frame-core wall structure system,floor system layout and component design,load value,reinforcing bars calculation and reinforcing bars of construction drawing. The internal forces and displacements on the structure under frequently occurred earthquake is analysed by structure software YJK,PKPM and MIDAS Building. EPDA&PUSH is adopted to analyse the internal forces and displacements on the structure under rarely occurred earthquake. In the case of meeting the requirements of codes and structure performance,amendments and recommendations are proposed to apply to structure design. Suggestions is proposed to solve the problems which is faced during the process of third-party audit and optimization

Effect of RF power on the structure and properties of ZnO∶Al films deposited by magnetron sputtering

采用RF磁控溅射技术以ZnO:Al2O3(2 wt%Al2O3)为靶材在石英玻璃衬底上制备多晶ZnO:Al(AZO)薄膜,通过XRD、AFM、AES以及Hall效应、透射光谱、折射率等手段研究了RF溅射功率(50~300 W)对薄膜的组织结构和电学,光学性能的影响。分析表明:所制备的AZO薄膜具有c轴择优取向,并且通过对不同功率下薄膜载流子浓度与迁移率的研究发现对于室温下沉积的AZO薄膜,晶粒间界中的O原子吸附是影响薄膜电学性能的主要因素。同时发现当功率为250 W时薄膜的电阻率降至最低(3.995×10-3Ω.cm),可见光区平均透射率为91%。Aluminum doped zinc oxide films are deposited by magnetron sputtering using a zinc oxide target doped with Al2O3(2 wt%.) with different RF powers on quartz substrate.The structural and compositional characteristics of the films are investigated by XRD,AFM,SEM,AES and XPS.respctively,while the electrical and optical properties of the thin films are studied by the Hall measurement and spectrophotometry,respectively.It has been found that all films deposited are c-axis preferred orientation perpendicular to the substrate with porous crystalline structure.The lowest resistivity obtained in this study is 3.9×10-3 Ω·cm for the film deposited at 250 W,and the average transmittance is 91% in the visible range.By comparing the samples deposited at various RF power,the oxygen absorption in the grain boundaries is the dominant factor which influences the electrical property of the AZO thin film

Structural,Electrical,and Optical Properties of Transparent Conductive Al-Doped ZnO Films Prepared by RF Magnetron Sputtering

室温下采用RF磁控溅射技术在石英衬底上制备了多晶ZnO:Al(AZO)透明导电薄膜,通过XRD,AFM,AES,Hall效应及透射光谱等测试研究了RF溅射功率、氩气压强对薄膜的结构、电学和光学性能的影响.分析表明:在最优条件下(溅射功率为250W,氩气压强为1.2Pa时),180nmAZO薄膜的电阻率为2.68×10-3Ω.cm,可见光区平均透射率为90%,适合作为发光二极管和太阳能电池的透明电极.所制备的AZO薄膜具有c轴择优取向,晶粒间界中的O原子吸附是限制薄膜电学性能的主要因素.Highly conductive transparent Al-doped zinc oxide(AZO)films with highly(002)-preferred orientation were successfully deposited on glass substrates at room temperature by RF magnetron sputtering.Optimization of deposition parameters was based on sputtering RF power and Ar pressure in the vacuum chamber.AZO films of 180nm with an electrical resistivity as low as 2.68×10-3 Ω·cm and an average optical transmission of 90% in the visible range were obtained at RF power of 250W and Ar pressure of 1.2Pa.The effect of chemisorption of oxygen on the grain boundary would capture electrons from conduction band and lead the formation of potential barriers among the crystallites,which will influence the electric property of the AZO thin films.The films have satisfactory properties of low resistance and high transmittance for application as transparent conductive electrodes in light emitting diodes(LEDs)and solar cells

重新认识“物理化学”课程的战略地位

鉴于当前某些高校化学系和生物学系在深化教改过程中对《物理化学》学科的性质、作用认识不足 ,以致于该课程面临被削弱、甚至被取消的境地 ,为此本文着重从理论高度和具体实践阐明该学科在经济振兴、科技腾飞、造才育帅中的战略地位

Promotional Effects of Ni and Mg on the Preferential Oxidation of CO over Carbon Nanotube-Supported Pt Catalyst

采用共浸渍法制备了nI和Mg促进的碳纳米管载PT催化剂(PT-nI-Mg/CnT),考察了其对CO优先氧化的催化性能.结果表明,PT,nI和Mg在CnT表面发生了相互作用,可能存在合金纳米粒子,从而显著改善了催化剂CO优先氧化的催化性能.添加适量nI可提高CO的转化率,而加入适量Mg则有助于改善CO2的选择性.当PT,nI和Mg同时负载时,优化后所得的催化剂5%PT-5%nI-5%Mg/CnT在100°C对富H2气体中CO选择氧化的转化率为100%,CO2的选择性为53.7%;该催化剂在140°C下连续反应24H,催化活性稳定.A carbon nanotube-supported Pt catalyst promoted by Ni and Mg(Pt-Ni-Mg/CNT) was prepared by the co-impregnation method for preferential oxidation of CO in H2-rich gas.The results of X-ray diffraction, H2 temperature-programmed reduction, transmission electron microscopy, and energy dispersive spectroscopy indicated that Pt interacted with Ni and Mg on CNT surface and might form alloy nanoparticles to some extents, which in turn markedly improved the catalytic performance of preferential oxidation of CO in H2-rich gas.The results of performance tests showed that CO conversion at low temperatures could be effectively enhanced by adding a proper amount of Ni, while the selectivity was improved by adding a proper amount of Mg.When Ni and Mg were added simultaneously, the 5%Pt-5%Ni-5%Mg/CNT catalyst showed CO conversion of 100% and selectivity for CO2 of 53.7% in a H2-rich stream at 100 °C.The catalytic performance did not change obviously during 24 h of run at 140 °C.国家重点基础研究发展计划(973计划;2009CB939804);福建省科技重大专项前期项目(2009HZ10102

Preliminary pharmacodynamic study of STS as an adjuvant ofthe chemotherapy drugs in Vitro and in Vivo

目的：研究硫代硫酸钠（STS）是否能在体内、外作为化疗药的辅药。方法：应用MTT法研究STS分别与AdM、MMC和CddP等6种抗癌单药合用时对bEl-7402和MgC80-3细胞的细胞毒性作用；应用小鼠肝癌腹水瘤（H22）模型判断STS与AdM等3种药合用时的抗癌疗效；20例人原发性肝癌病人肝动脉插管化疗（AdM、MMC和CddP）前30MIn静脉推注STS（125~25g/M2）考察STS作为化疗药辅药的作用。结果：除了CddP以外，STS（500μg/Ml）与AdM等5种抗癌单药合用时对抗癌药的抗肿瘤细胞活性无明显影响（P＞005）。当不同剂量STS（350Mg/kg、35Mg/kg）分别与AdM（6Mg/kg）、MMC（14Mg/kg）和CddP（45Mg/kg）合用或仅这3种化疗药合用时，这3组腹水瘤小鼠的存活期比对照组者显著延长（P＜0001），但3组之间无明显区别（P＞005）。人肝癌肝动脉插管化疗时，STS与AdM、MMC和CddP抗癌药合用，肝癌治疗总有效率达60%，且可减少70%病人的恶心、呕吐等副作用。结论：在体内，低浓度的STS（125~25g/M2）可与AdM，MM？Objective: To study whether sodium thiosulfate (STS) can be used as an adjuvant of the chemotherapy drugs. Methods: The cytotoxicity of STS combined with ADM, MMC, CDDP, 5Fu, MTX and VCR (1PPC/ml) respectively on the cells of BEL7402 and MGc803 was studied by MTT test in vitro.The ascitic hepatoma (H22) in mice were adopted to determine the anticancer effect of STS adjuvant of ADMMMC and CDDP.Being treated with STS(12525 g/m2) 30 min before drug administration,20 patients of hepatocellular carcinoma (HCC) were carried on transcatheter arterial chemoembolization with ADMMMC and CDDP to observe the adjuvant effect of STS. Results: The anticancer activity of ADM and the other agents respectively adjuvant with STS (500 g/ml) were not obviously influented (P >005), but that for CDDP was influented .It was proven that the survival time of ascitic hepatoma treated with ADM (6 mg/kg),MMC (14 mg/kg) and CDDP (45 mg/kg) alone or three drugs in addition to STS (350 mg/kg,35 mg/kg) respectively was not significantly different (P >005).The survival time of each groups was significantly longer than that of control group (P< 0001).It have also been proven that STS adjuvant of ADM, MMC and CDDP was not decreased the chemotherapry effect (RR=60%) by transcatheter arterial chemoembolization in HCC, simultaneously it alleviated the nausea and vomiting in 70%patients. Conclusion: The study provides evidence that in vivo, STS (12525 g/m2) can be used as an adjuvant of chemotherapy drugs,such as ADM,MMC, including CDDP, and so on , for decreasing nausea and vomiting effects, espacially for transcatheter chemotherapy .福建省“八五”肝癌攻关课

Comparative studies on carbon storage and litterfall dynamics between secondary and primary mangrove communities in Zhanjiang,Guangdong Provinces,China

通过群落调查、异速生长法计算以及样品测定,分别对2010~2011年期间广东湛江高桥红树林生态系统中次生桐花树和原生木榄群落的植物和土壤储量进行了计算,并利用掉落物收集筐对掉落物动态进行了比较研究。研究结果表明,高桥桐花树与木榄群落的植物碳库(以C计,下同)分别为(51.16±12.06)x106g/HM2和(38.52±6.94)x106g/HM2,地上部分明显高于地下部分;土壤碳库分别为(111.86±7.96)x106g/HM2和(106.13±11.12)x106g/HM2,明显高于植物碳库;次生与原生红树林群落的总碳库没有差异(P>0.05)。桐花树和木榄群落的年均掉落物产量分别为556.00 g/M2和971.13 g/M2,均以凋落叶居多,但原生木榄群落的掉落物产量明显高于次生桐花树群落(P 0.05).The mean annual litterfall production for the B.gymnorrhiza community was 971.13 g DW / m2,significantly higher than that of the A.corniculatum community( 556.00 g / m2,P < 0.001).In conclusion,the carbon pools in the secondary A.corniculatum community were comparable with those for the primary B.gymnorrhiza community,but much lower than those for the primary mangrove communities in the tropical regions.国家自然科学基金重点项目(30930017); 国家海洋公益性行业科研专项经费项目(200905009;201305021

Promotional Effects of Ni and Mg on the Preferential Oxidation of CO over Carbon Nanotube-Supported Pt Catalyst

A carbon nanotube-supported Pt catalyst promoted by Ni and Mg (Pt-Ni-Mg/CNT) was prepared by the co-impregnation method for preferential oxidation of CO in H-2-rich gas. The results of X-ray diffraction, H-2 temperature-programmed reduction, transmission electron microscopy, and energy dispersive spectroscopy indicated that Pt interacted with Ni and Mg on CNT surface and might form alloy nanoparticles to some extents, which in turn markedly improved the catalytic performance of preferential oxidation of CO in H-2-rich gas. The results of performance tests showed that CO conversion at low temperatures could be effectively enhanced by adding a proper amount of Ni, while the selectivity was improved by adding a proper amount of Mg. When Ni and Mg were added simultaneously, the 5%Pt-5%Ni-5%Mg/CNT catalyst showed CO conversion of 100% and selectivity for CO2 of 53.7% in a H-2-rich stream at 100 degrees C. The catalytic performance did not change obviously during 24 h of ran at 140 degrees C

Preventive effect and mechanisms of 3,3-diindolylmethane on oxidative stress induced by hydrogen peroxide in HaCaT cells

目的:探究3,3'-二吲哚甲烷(dIM)对过氧化氢(H_2O_2)诱导人角质形成细胞(HACAT)氧化应激作用的预防效应及可能机制。方法:体外培养HACAT细胞,用H_2O_2建立氧化应激模型。采用CCk-8法检测不同浓度(1~20μMOl/l)dIM对HACAT细胞生长的抑制作用;流式细胞术检测dIM作用前后细胞内活性氧自由基(rOS)含量的变化;WESTErn blOT检测不同浓度dIM(0、5、10μMOl/l)对HACAT氧化应激相关蛋白核因子(nf-κb)和丝裂原活化蛋白激酶(MAPkS)磷酸化表达水平的影响。结果:成功建立了HACAT氧化应激模型。CCk-8法研究结果显示1-10μMOl/l dIM对HACAT细胞无明显毒性作用(P>0.05);流式细胞术检测结果表明10μMOl/l dIM预处理可有效预防由H_2O_2诱导的HACAT内rOS产生(P0.05).Flow cytometry results indicated that pretreatment with DIM(10 μmol/L) could inhibit the level of intracellular ROS(P<0.05).With increasing concentration of DIM,the levels of p-p38-MAPK,p-JNK and p-NF-κB were significantly depressed.CONCLUSION:DIM could protect HaCaT cells from H_2O_2-induced oxidative stress via suppressing production of ROS levels and down-regulating the expression of NF-κB and members of MAPKs.DIM might be used as an effective drug to treat or reduce oxidative stressmediated injury in skin cells.国家自然科学基金青年基金(81101562); 广东省科技计划项目(2012B060300005); 广东省自然科学基金(S2012010009633