A comparative characteristic of the qualitative and quantitative composition of the microbiocenosis of palatine tonsils in patients with chronic tonsillitis and in healthy individuals was established. Seeding density biotope of patients with chronic tonsillitis averaged lg (6,2 ± 0,4) CFU/g against average colonization by microorganisms of tonsils in healthy persons (4,6 ± 0,4) CFU/g. Moreover, it was revealed that the use of high-energy semiconductor laser leads to consistent and pronounced positive effect on microbiological parameters that were studied. The stability of the results that was achieved was confirmed microbiologically in the dynamic monitoring of patients. In 49,9 % of patients that received conservative therapy, the degree of dysbiotic manifestations had worsened. The obtained indexes statistically did not differ from the microbial communities of the mucous membranes of healthy individuals that were managed in 63,3 % patients who underwent pinpoint laser blast on the tonsils.A comparative characteristic of the qualitative and quantitative composition of the microbiocenosis of palatine tonsils in patients with chronic tonsillitis and in healthy individuals was established. Seeding density biotope of patients with chronic tonsillitis averaged lg (6,2 ± 0,4) CFU/g against average colonization by microorganisms of tonsils in healthy persons (4,6 ± 0,4) CFU/g. Moreover, it was revealed that the use of high-energy semiconductor laser leads to consistent and pronounced positive effect on microbiological parameters that were studied. The stability of the results that was achieved was confirmed microbiologically in the dynamic monitoring of patients. In 49,9 % of patients that received conservative therapy, the degree of dysbiotic manifestations had worsened. The obtained indexes statistically did not differ from the microbial communities of the mucous membranes of healthy individuals that were managed in 63,3 % patients who underwent pinpoint laser blast on the tonsils