This research aims to produce super brood of catfish who has a chromosome structure 4N (tetraploid) which will be used as a basis to generate triploid catfish. The specific objective of research was to determine the proper method to produce tetraploid individual catfish, knowing the character of the growth of catfish tetraploid, and knowing the character of morphometric and meristik catfish tetraploid. The study was conducted in Fisheries Laboratory of Lampung State Polytechnic and planned to be carried through three stages: Formation of catfish tetraploid by applying the method of temperature shock, fish ploidy analysis of treatment outcome, and the tetraploid selection. Special Purpose study is to get the population of catfish that have to be tetraploid or 4N ploidy and produce brood catfish putatively tetraploid. Target outcomes study the third year that the main candidates tetraploid. The results of research conducted showed tetraploid individuals found in all treatments given temperature shock to the level of an individual percentage of catfish tetralploid between 5-30%. The determination of ploidy levels in catfish is done using a calculation of the number nucleolus. Individual catfish nucleolus tetraploid have maximum 5-6 pieces.Keyword: catfish, tetraploid, engineered chromosome, genetic
