Sustainable aquaculture requires the reduction of fish meal use in diets, by replacing it with ecofriendly ingredients, as that vegetal feedstuff not desirable for human consumption. However,
these pose challenges due to the presence of antinutritional factors. The supplementation with
some supplements, like exoenzymes and antioxidants, has shown potential to reduce their
nutritional drawbacks. The solid-state fermentation (SSF) of agro-industrial wastes may be used
to obtain such compounds. The goal of this work was to test the applicability of an SSF-extract,
obtained through the SSF of an optimized mixture of three agro-industrial wastes with Aspergilus
ibericus (30% EGM, 36% VTS, 34% EOP), determined previously with a simplex-centroid mixture
design. The activity of this extract was measured to be xylanase: 1866.9 U/g (lyophilized extract);
cellulose: 1563.6 U/g; β-glucosidase: 736.455 U/g, and total antioxidants: 439.4 μmol Trolox
equivalents/g lyophilized extract; total phenols: 28.1 mg caffeic acid equivalents/g lyophilized
extract. The efficiency of this extract as a feed additive in plant-based diets evaluated by an in
vitro digestibility trial, analyzing its efficiency to improve the release of pentoses and antioxidants
during digestion. The extract was used to supplement diets for Dicentrarchus labrax, with
increasing levels of extract: 0, 4 and 8 U cellulase/g diet. Dietary supplementation with the extract
led to the increase of pentose release, during alkaline digestion. The effect of the
supplementation with the SSF extract on the release of antioxidants and phenols compounds are
being evaluated and will be presented.Project SPO3 (ref. POCI-01-0145-FEDER-030377; FEDER-Operational Programme
Competitiveness and Internationalization and FCT); project InovFeed (ref. MAR-02.01.01-FEAMP0111; Programa Operacional Mar2020); BioTecNorte (NORTE-01-0145-FEDER-000004; ERDF
under the scope of Norte2020-Programa Operacional Regional do Norte)info:eu-repo/semantics/publishedVersio
