Determination of volatile aroma compounds in fresh Origanum vulgare and Hyssopus officinalis: Headspace GC/FID/MS profile

Abstract

Origanum vulgare and Hyssopus officinalis are important aromatic and medicinal plants. The chemical composition of the volatile aroma compounds was defined in the fresh aerial part of Origanum vulgare (f-O) and Hyssopus officinalis (f-H), collected in Valbona (Albania). The analysis were made by gas chromatography – mass spectrometry (GC/FID/MS) on HP5-ms column and equipped with automated headspace (HS) system. 0,3 g fresh plant material (homogenized samples from flower, leaf and stem) was put in sealed vials, heated (80 ͦC) and the gas phase was investigated. Total of 21 individual volatile aroma compounds were identified in f-O sample, 14 monoterpenes (78.35%) and 7 sesquiterpenes (3.03%). Dominant components were monoterpene hydrocarbons: sabinene (55.05%), trans-β-ocimene (5.19%) and γ-terpinene (4.05%), followed by cis-sabinene hydrate, α-terpinene and β-pinene. In f-H sample were identified 16 individual volatile aroma components, 11 monoterpenes (84.51%) and 5 sesquiterpenes (0.29%). The major components were monoterpene hydrocarbons: β-pinene (48.66%) and cispinocamphone (29.77%), followed by α-pinene and α-thujen

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