Amado, Isabel

Cerqueira, Miguel A.

Cunha, Rosiane

Gonçalves, Catarina

Martins, Artur J.

Pastrana, Lorenzo

Vicente, A. A.

English

Universidade do Minho: RepositoriUM

6th ICFD  April 2-4, 2019Granada, SpainP a g e | 2 3 7378/239. BIOACCESSIBILITY OF BETA-CAROTENE LOADED OLEOGELS(1)Goncalves, C., (2)Martins, A., (3)Amado, I., (1)Pastrana, L., (4)Cunha, R., (2)Vicente, A., (1)Cerqueira, M.(1)Food Processing & Nutrition Group. International Iberian Nanotechnology Laboratory. Portugal., (2)Centre of Biological Engineering. University of Minho. Braga. Portugal., (3)Analytical and Food Chemistry Department. University of Vigo. Ourense. Spain., (4)Department of Food Engineering. University of Campinas. Campinas. Brazil.Introduction Oleogels are able to provide a healthier alternative for saturated fat replacement in foods, while pro-viding tailor-made nutritional, structural and textural properties. Despite the great number of works on the development and characterization of this kind of systems using different materials, their per-formance as carriers of bioactive compounds, and their behavior under gastrointestinal conditions has been scarcely studied.Objective In vitro study of the bioaccessibility of beta-carotene loaded in two distinct edible oleogels prepared through single- (beeswax) or multi-component (sterol mixture) gelation. Methodology High Oleic Sunflower Oil (HOSO) was structured with 8% (w/w) of gelator (beeswax or a mixture of gamma-oryzanol and beta-sitosterol (60:40 ratio). Both oleogels were loaded with 0.1% (w/w) of beta-carotene. In vitro digestion of oleogels was performed in three stages (mouth, stomach and small intestine) following the INFOGEST standard protocol. A visual inspection of the digested oleo-gels and fluorescence microscopy were performed. The amount of beta-carotene was quantified by HPLC. The cytotoxicity of digested oleogels was evaluated by monitoring transepithelial electrical resistance (TEER) of Caco-2 + HT-29-MTX monolayers, up to 4h of contact.Main findings Oleogels were evaluated in terms of their textural properties and the results showed that sterol-based oleogels have an average hardness approximately 4-fold higher than beeswax oleogels. In vitro di-gestion demonstrated that both oleogels showed structural disintegration after the gastric and intes-tinal phase. The bioacessibility of beta-carotene was low, however the amount of bulk lipid could be adjusted, in order to improve the bioaccessibility. No cytotoxicity was detected along the experiment.Conclusion This study demonstrates that the INFOGEST standard protocol is suitable for the evaluation of in-vi-tro digestion of oleogels. The acquired bioaccessibility of beta-carotene in the tested formulations was found to be low. It was demonstrated that oleogels might have potential on the development of functional foods. Key words Beta-carotene, bioaccessibility, bioactive, digestion, oleogel 

Bioaccessibility of beta-carotene loaded oleogels

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Bioaccessibility of beta-carotene loaded oleogels

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Universidade do Minho: RepositoriUM