[Excerpt] The ways proteins compete for the surface of biomaterials and
change conformation are believed to be important for the host response
to implants. It is possible to elucidate information on
packing and any induced conformational change by making use of
different fluorescence techniques on fluorescently labelled proteins.
Employing probe-probe resonance energy transfer (RET) allows
inter and intra protein interactions to be distinguished. Homo resonance
energy transfer (hRET) avoids many problems with having
two different probes and means that labelling and subsequent purification
can be done in one step. [...]Portuguese Foundation for Science and
Technology, project PROTEOLIGHT (PTDC/FIS/68517/2006)
and J.B. grant SFRH/BPD/17584/2004. European Union NoE
EXPERTISSUES (NMP3-CT-2004-500283) and European Union
FP6 STREP project HIPPOCRATES (NMP3-CT-2003-505758).info:eu-repo/semantics/publishedVersio