Couto, Márcia R.

Rodrigues, Joana Lúcia Lima Correia

Rodrigues, Lígia M.

English

Universidade do Minho: RepositoriUM

Engineering a biosynthetic pathway for high-value glycosaminoglycans production

Engineering a biosynthetic pathway for  high-value glycosaminoglycans production Márcia R. Couto*, Joana L. Rodrigues, Lígia R. Rodrigues Centre of Biological Engineering, University of Minho, Braga, Portugal, *e-mail: marciacouto93@gmail.com MIT Portugal 2018 Annual Conference, Lisbon, October 1st, 2018 pUC57_ugdh ugdh amplification Expected ~1.5 kb pSP-GM1 (shuttle vector) Extraction Digestion & Ligation pSP-GM1_ugdh Positives ~2kb Colony PCR (E. coli) Plasmid extraction & sequencing POSITIVES CONFIRMATION Transformation in S. cerevisiae - control Colony PCR S. cerevisiae Y02321 Colony PCR S. cerevisiae CEN.PK2-1C Positives ~2kb Positives ~2kb Transformation in E. coli UDP-glucose dehydrogenase UDP-N-acetylglucosamine 4-epimerase Chondroitin polymerase       PRELIMINARY RESULTS UDP-glucose dehydrogenase UDP-N-acetylglucosamine 4-epimerase Chondroitin polymerase   GOAL & METHODOLOGY    Glycosaminoglycans (GAGs) Natural polysaccharides with important cosmetic and medical applications  Hyaluronic acid  Heparan  Chondroitin Sulfate  INTRODUCTION     [1] Chondroitin Sulfate Market - Global Industry Analysis, Size, Share, Growth, Trends, and Forecast 2017-2025, Transparency Market Research, https://www.transparencymarketresearch.com/chondroitin-sulfate-market.html (visited on 10st September 2018) [2] He W et al. (2015) Production of chondroitin in metabolically engineered E. coli, Metabolic Engineering, 27:92-100.  REFERENCES Osteoarthritis supplement Global Market US$457.7 M (2025) CAGR of 3.3% (2017-2025)[1] GAG-like polysaccharides E.g. Pasteurella multocida Escherichia coli K4: [2] Shark fins and cartilage 1 2 3  Design and engineer an artificial pathway for the production of chondroitin in Saccharomyces cerevisiae 1 2 3   Enzymatic activity evaluation Pathway assembly 

http://repositorium.sdum.uminho.pt/bitstream/1822/57743/1/document_49412_1.pdf

Engineering a biosynthetic pathway for high-value glycosaminoglycans production

Abstract

Similar works

Full text

Available Versions

Universidade do Minho: RepositoriUM

Universidade do Minho: RepositoriUM