2nd International Conference on Protein Stabilisation - From Molecular Interpretation to Bio-Industrial Applicationsinfo:eu-repo/semantics/publishedVersio

Dourado, Fernando

Gama, F. M.

Mota, M.

Vasco, Pedro

Universidade do Minho: RepositoriUM

2 .wl International Conference on Protein Stahilisation/Biomolecule Stabilisation - Lisbon, 9-12 April 2000 Properties and Recycling of Covalently Immobilized Glycanases used for Aqueous Enzyme Assisted "Rosa Mosqueta." Oil Extraction F. Dourado, P. Vasco, F. M. Gama and M.Mota Departamenco de Engenharia Bio/6gica, Universidade do Minho, Largo do Pafo,TBFQ -lnstituto de Biotecnologia e Qufmica Fina4719 Braga Codex, Portugal Keywords: Enzyme recycling, immobilization, oil extraction In order to reuse enzymes after enzyme reaction and to enhance their stabil-ity, the immobilization of enzymes using an insoluble carrier has been widely studied. Cellulase was immobilized on Eudragit L, that is reversibly soluble depending on the pH of the medium, according to procedure described by Taniguchi et al [1]. It has been demonstrated that this method allows for improved stability, particularly at high temperatures, without major loss of specific activity. Also, it has been shown that the derivatised proteins have the adsorption equilibrium switched towards the liquid phase, thus making recy-cling possible. The enzyme assisted aqueous oil extraction may be useful to circumvent problems associated to the use of organic solvents. Commercial glycanases, used in this process to solubilize the cell wall polymers, are usu-ally very stable enzymes, and, even if their price is lower and lower, its reuti-lization is still worth. As these enzymes adsorb on to the lignocellulosic mate-rials where they perform their hydrolytic action, most of the protein remains attached to the solid phase after processing, and is therefore inaccessible for reusing. The experimental flow-sheet to be tested with the "Rosa Mosqueta" seeds included a) a milling step; b) addition of a pH 5,5 buffer plus immobi-lized enzymes (Celluclast) in a ratio of lg protein / 100 g seeds, in order to hydrolyse the cell walls; c) water I azeotropic ethanol oil extraction; d) dry-ing of the seeds before soxhlet hexane extraction, in order to quantify the residual oil. After the enzymatic treatment the enzyme is recovered by cen-trifugation and filtration, followed by acidification of the medium to precipitate the insoluble carrier of the enzyme. [1] Taniguchi et al. Biotechnot. and Bloeng. ;M, 1092-1097, 1989. posters P61 Ill• Centre for Biological and Chemical Engineerin 2 Instituto Superior Tecnico nd International on Protein Conference Stabilisation Biomolecule Stabilisation From Molecular Interpretations to Bio-Industrial Applications Calouste Gulbenkian Foundation, Lisbon, 9-12 April 2000 

Properties and recycling of covalently immobilized glycanases used for aqueous enzyme assisted Rosa mosqueta oil extraction

http://repositorium.sdum.uminho.pt/bitstream/1822/54940/1/document_3491_1.pdf

Properties and recycling of covalently immobilized glycanases used for aqueous enzyme assisted Rosa mosqueta oil extraction

Abstract

Similar works

Full text

Available Versions

Universidade do Minho: RepositoriUM