We here report a double-layer microfluidic device for detecting and identifying secreted extracellular vesicle populations, among them exosomes, from single long-term cultured cells using multi-color Total Internal Reflection Fluorescence Microscopy (TIRFM). Using a custom-developed MATLAB script for image processing we achieved quantitative population analysis of vesicles secreted from single Michigan Cancer Foundation (MCF)-7 cells cultivated for 2-5 day
