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Animal facility videoendoscopic intubation station: tips and tricks from mice to rabbits

Abstract

Endotracheal intubation of laboratory animals is a common procedure shared by several research fields for different purposes, such as mechanical ventilation of anaesthetized animals, instillation of cytotoxic nanoparticles, infectious agents or tumour cells for induction of disease models, and even for diagnostic and therapeutic purposes. These different research purposes, achieved in different animal models, require technical expertise and equipment that suits every research need from animal facilities. In this short report we propose a videoendoscopic intubation station that could be shared among the most common laboratory animals, namely the mouse, rat, guinea pig and rabbit, from neonates to adult animals. This report aims to contribute to the reduction of animals excluded from experiments due to false paths during direct and blind intubations and to the refinement of procedures by replacing surgical approaches such as tracheotomy.Project ON.2 SR&TD Integrated Program (NORTE-07-0124-FEDER-000017), co- funded by the North Portugal Regional Operational Program (ON.2-O Novo Norte), under the National Strategic Reference Framework, through the European Regional Development Fund (ERDF). This work was also supported by FEDER funds through the Operational Programme Competitiveness Factors – COMPETE and National Funds through FCT – Foundation for Science and Technology under the project POCI-01-0145-FEDER-007038. A Miranda was supported by an FCT grant (SFRH/BD/52059/2012)info:eu-repo/semantics/publishedVersio

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