The proposed research project is based on the hypothesis that long-term low and moderate alcohol protects the liver from progression to NAFLD. The general objective of this thesis is to investigate the in vivo and in vitro effects of alcohol on NAFLD induced by HFD-fed rat model. The specific objectives of this thesis are (i) To elucidate the serum and histopathological changes observed by alcohol treatments on in vivo model, (ii) To elucidate the possible mechanism(s) of action of low and moderate alcohol on controlling HFD-induced NAFLD in the liver, (iii) elucidate the possible mechanism(s) of action of low and moderate alcohol on controlling FFA-induced NAFLD in HepG2 cells, (iv) evaluating the effect of alcohol on FFA-induced NAFLD in HeLa cells (LKB1-deficient cells) (v) evaluating the effect of alcohol on FFA-induced NAFLD in HepG2 cells in absence of AMPK, (vi) evaluating the effects of alcohol on FFA-induced NAFLD in HepG2 cells in absence of SIRT1
