Enhancing the enzymatic saccharification of whole slurry from autohydrolyzed Eucalyptus globulus wood by supplementation with a recombinant carbohydrate-binding module
Microbiotec'17 - Congress of Microbiology and Biotechnology 2017Background: Lignocellulosic biomass has a recognised potential as a sustainable platform for the production of biofuels and other biochemicals. However, lignin residues and other inhibitory compounds resulting from lignocellulosics pretreatment affect the digestibility of resulting whole slurries. The addition of synergistic proteins that can cooperate with cellulases is an emerging strategy for enhanced lignocellulosics hydrolysis. Carbohydrate-binding modules (CBMs) have been shown to improve the enzymatic hydrolysis of pure cellulose models but their effect on the enzymatic hydrolysis of lignocellulosic materials has not yet been evaluated. Thus, in this work, the potential synergistic effect of a family 3 CBM on the enzymatic saccharification of a pretreated lignocellulosic biomass was studied for the first time. [...]C. Oliveira and D. Gomes acknowledge support from Fundação para a Ciência e a Tecnologia (FCT), Portugal (grants SFRH/BPD/110640/2015 and SFRH/BD/88623/2012, respectively). This study was supported by the FCT under the scope of the GlycoCBMs Project PTDC/AGR-FOR/3090/2012–FCOMP-01-0124-FEDER-027948 and the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684) and BioTecNorte operation (NORTE-010145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte.info:eu-repo/semantics/publishedVersio
