Recently, renewed interest has arisen in silver nanopar@cles for biomedical devices because of their high surface energy, enhanced physicochemical and biological proper@es and extremely large surface area, which provides beAer contact with microorganisms. Atmospheric plasma is an alterna@ve and cost- compe@@ve method to wet chemical nanopar@cles deposi@on methods, avoiding the need of toxic solvents, expensive vacuum equipment and allowing con@nuous and uniform processing of material surfaces. However, there are no reports on the size and @me-dependent an@microbial, physical and chemical surface effects of the silver nanopar@cles immobilized on plasma func@onalized polymers. Thus, the purposes of this study were: (i) the silver nanopar@cle size and aging effects aCer 30 days on the an@microbial ac@vity aCer deposi@on onto DBD plasma-treated polyamide 6,6 fabrics, and (ii) the aging effect on the physico-chemical binding mechanism between different sized silver nanopar@cles and the plasma treaded polyamide 6,6. Five different in size commercial silver nanopar@cles have been employed (10, 20, 40 60 and 100 nm).This work was funded by Portuguese Founda@on for Science and Technology FCT/MCTES (PIDDAC) and co-financed by European funds (FEDER) through the PT2020 program, research project M-ERA-NET/0006/2014 and COMPETE program through FCT within the scope of the project POCI-01-0145-FEDER-007136 and UID/CTM/00264.info:eu-repo/semantics/publishedVersio