EUROBIOFILMS 2017 - 5th European Congress on Microbial BiofilmsS. epidermidis is one of the most common causes of medical device-related infections, being
associated with increased patients’ morbidity but also with massive additional financial
burden. Hence, several efforts have been made to identify S. epidermidis virulence
determinants, including the mechanisms behind biofilm formation and antibiotics tolerance.
However, the great majority of these studies were performed using artificial media, which
composition does not reflect in vivo conditions, and in the absence of host factors reducing
the clinical relevance of those findings. Thus, the aim of this work was to evaluate the
influence of host factors present in human plasma on S. epidermidis growth rate, biofilm
formation capacity and tolerance to antibiotics. The presence of human plasma (5 to 20%) in
the culture medium (Tryptic Soy Broth) significantly decreased both growth rate and biofilm
formation ability. The greater the plasma concentration (20%), the greater the inhibitory
effect observed (2 log10 reduction in growth and 3-fold less biofilm biomass). Interestingly,
different results were obtained when the brand of the medium was changed. Thus, due to
the inherent variability of complex media, chemically defined medium (CDM) was used
instead. Respecting the bacterium growth rate, once more, the greater the plasma
concentration, the greater the inhibition observed although with CDM the impact was less
pronounced (1 log10 reduction in the presence of 20% of plasma). However, different from
what was observed with TSB, no differences were found on biofilm formation capacity. In
addition, our data showed that human plasma decreased the effect of vancomycin as 1.3
log10 reduction was observed in the absence of plasma and only 0.5 in the presence of 20%
of plasma. Overall, our results highlight the importance of introducing host factors in in vitro
assays in order to better mimic in vivo conditions consequently increasing the clinical
significance of the results obtained.info:eu-repo/semantics/publishedVersio