Nowadays, the rising of multi resistant bacteria due to the misuse of
antibiotics, together with the lack in the development of new antimicrobial
molecules, represents a serious threat to Medicine. Many of these multi
resistances bacteria are pathogens responsible for nosocomial infections
causing problem in Human health and care.This work addresses the
problematic of treating chronic wounds, lacerations and infected burns. In
these types of infections, bacteria colonize the tissues and form biofilms,
conditioning the effectiveness of antibiotics.Based on this reality, the
development of news approaches to treat multi resistance organisms is
essential. A combination of two antimicrobial solutions, bacteriophages
(phages) and honey, is suggested herein. Bacteriophages (phages) are
natural bacteria predators that specifically recognize hosts, destroying it.
Honey is also able to destroy bacteria, but also to degrade biofilms, to
improve the healing process, promoting tissue regeneration, and to
decrease the inflammation. Both are efficient against antibiotic resistant
microorganisms, including those present in chronic wounds. To date there
is no study about this combinatorial therapy.In this study, the anti-biofilm
effect of the combination of a Portuguese honey together with wellstudied
lytic phages was enhanced, in Escherichia coli and
Pseudomonas aeruginosa biofilms relatively to the same antimicrobials
used individually. This result was confirmed by a decrease in the total
biomass, and by a reduction, in average, of more than 3 log of cultivable
cells
