The use of auxotrophies as a tool for genetic modification of microbial strains is widely employed in microbiology laboratories. However, there are some recognized problems associated with their use, such as the fact that it is unclear how much supplementation is required for each auxotrophy and microorganism1. Among the auxotrophies, one of the most used is that for uracil. In this case, the strains require the supply of exogenous uracil to compensate their nutritional deficiency. However, in some studies exogenous uridine is used instead of uracil.
The flavinogenic A. gossypii Agura3 strain is unable to grow at the same level of the parent strain when the medium is supplemented with uracil alone, being necessary the addition of uridine to overcome this effect2. A similar effect was also reported for other flavinogenic fungi, such as Pichia guilliermondii (Candida guilliermondii) 3.
Given the importance of these uracil auxotrophic strains, it is of extreme pertinence to elucidate the determinants behind this inadequacy of uracil to rescue the growth of flavinogenic uracil auxotrophs. Therefore, in this work we investigated the capability of the intermediates of the salvage pathways of pyrimidine ribonucleotides to rescue the growth of A. gossypii Agura3 and verified that uridine is the most adequate for growth supplementation. Based on these results, a hypothesis identifying the potential limiting step of this pathway in A. gossypii is proposed.Acknowledgments to FCT, Portugal: strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684) (Post-Doc fellowship to TQ Aguiar), BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by FEDER under the scope of Norte2020 - Programa Operacional Regional do Norte, and PhD grant PD/BD/113812/2015 to R Silva
