Essential oils (EOs) extracted from Lavandula luisieri and Cymbopogon citratus were tested for their antifungal activity against ten clinical isolates of dermatophytes isolated from cases of tinea pedis. Inhibition of conidial germination and antifungal drug/EO combination assay were tested on two ATCC reference strains of Trichophyton rubrum and Trichophyton mentagrophytes. EOs were characterised by high amount of oxygenated monoterpenes in their composition. Strong antifungal activity was observed for the majority of clinical strains, and fungicidal activity was demonstrated. Positive interaction between L. luisieri EO combined with terbinafine was observed against terbinafine-resistant strain (Tr ATCC MYA-4438). Significative reduction of the germination was observed above 100 g mL1. Both oils were safe to macrophage mammalian cells at tested concentration. This study describes the antifungal activity of L. luisieri and C. citratus EOs against dermatophytes, which could be useful in designing new formulations for topical treatments.This work was supported by Portuguese Foundation for Science and Technology (FCT) [grant number PEST-C/FIS/UI607/2013], [grant number UID/BIO/04469/2013], [grant number PEst-OE/EQB/LA0023/2013] and European Community fund FEDER [grant number FCOMP-01-0124-FEDER-007025], [grant number RECI project: FCOMP-01-0124-FEDER-027462].The authors thank the FCT Strategic Project PEst-OE/EQB/LA0023/2013. Nicolina Dias acknowledges the project “Consolidating Research Expertise and Resources on Cellular and Molecular Biotechnology at CEB/IBB”, Ref. FCOMP-01-0124-FEDER-027462