Bugianesi, Elisabetta

Hannes, Hagstrom

Henning, Gronbek

Holger, J Moller

Konstantin, Kazankov

Per, Stal

Ramy, Younes

Rosso, Chiara

English

Institutional Research Information System University of Turin

Macrophage markers do not add to the prediction of liver fibrosis by transient elastography in patients with non-alcoholic fatty liver diseaseKonstantin Kazankov1, Chiara Rosso2, Ramy Younes2, Hannes Hagstrom3,4, Holger Jon Moller5, Per Stal6,7, Elisabetta Bugianesi2, Henning Gronbak1. 1Aarhus University Hospital, Department of Hepatology and Gastroenterology, Aarhus N, Denmark; 2University of Turin, Division of Gastroenterology and Hepatology, Department of Medical Sciences, Turin, Italy; 3Karolinska University Hospital, Unit of Hepatology, Department of Upper GI, Stockholm, Sweden; 4Karolinska Institutet, Clinical Epidemiology Unit, Department of Medicine, Solna, Stockholm, Sweden; 5Aarhus University Hospital, Department of Clinical Biochemistry, Aarhus N, Denmark;6Karolinska University Hospital, Department of Upper GI, Stockholm, Sweden; 7Karolinska Institutet, Department of Medicine, Huddinge, Stockholm, SwedenBackground and Aims: Fibrosis staging by non-invasive tests is essential in non-alcoholic fatty liver disease (NAFLD). Measurement of liver stiffness by transient elastography (TE) is a well-established method for liver fibrosis assessment in NAFLD. We have previously shown that macrophage marker sCD163 is an independent predictor for fibrosis in NAFLD, and that combining sCD163 with e.g. NAFLD Fibrosis score improves the precision. In the present study we testedwhether the combination of macrophage markers and TE improves fibrosis prediction compared to the individual parameters.Method:We measured macrophage markers soluble CD163 (sCD163) and mannose receptor (sMR) using in-house ELISA assays in two independent cohorts from Italy (n = 141) and Sweden (n = 70) with biopsy-proven NAFLD and available TE.Results: In the Italian cohort, TE and sCD163 showed similar moderate associations with liver fibrosis (rho = 0.56, p < 001 and rho = 0.45, p < 0.001, respectively). TE had an area under the Receiver Operating Characteristics curve (AUROC) (with 95% CI) for F2 fibrosis: 0.79 (0.72–0.87), F3: 0.81 (0.73–0.89), F4: 0.95 (0.90–1.0). sCD163 also predicted fibrosiswell [F2: 0.71 (0.63–0.80), F3: 0.82 (0.74–0.90), F4: 0.89 (0.76–1.0)]. However, combining sCD163 and TE did not improve the AUROCs significantly [F2: 0.79 (0.72–0.87), F3: 0.85 (0.78–0.92), F4: 0.97 (0.93–1.0)]. In the Swedish cohort, TE showed a closer association with fibrosis (rho = 0.73, p < 0.001) than sCD163 (rho = 0.42, p < 0.001) and sMR (rho = 0.39, p < 0.001). TE predicted fibrosis well [F2: 0.88 (0.80–0.97), F3: 0.90 (0.83–0.97), F4: 0.87 (0.78–0.96)], whereas sCD163 did not (best AUROC 0.75). sMR showed a better prediction [F2: 0.68 (0.56–0.81), F3: 0.82 (0.71–0.92), F4: 0.79 (0.66–0.93)], but the addition of sMR did not further improve the prediction of fibrosis by TE.Conclusion: In these cohorts of NAFLD patients, TE was superior to macrophage markers for fibrosis prediction and in contrast to our hypothesis the addition of these markers to TE did not improve its predictive capability.

Macrophage markers do not add to prediction of liver fibrosis by transient elastography in patients with non-alcoholic fatty liver disease

https://iris.unito.it/bitstream/2318/1752420/1/Kazankov%20EASL%202020.doc

Macrophage markers do not add to prediction of liver fibrosis by transient elastography in patients with non-alcoholic fatty liver disease

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