In this work, biosurfactant production by Pseudomonas aeruginosa and Bacillus subtillis strains was optimized using low-cost substrates. The highest biosurfactant production (3.2 g/L) by the P. aeruginosa strain was obtained using a culture medium containing corn steep liquor (CSL) (10% (v/v)) and molasses (10% (w/v)), whereas the best biosurfactant production by the B. subtillis isolate (1.3 g/L) was obtained using a culture medium consisting of 10% (v/v) of CSL. Subsequently, for the B. subtillis strain, the effect of different metals (iron, manganese and magnesium) on biosurfactant production was evaluated. When the culture medium CSL 10% was supplemented with the optimum concentration of those metals simultaneously, the biosurfactant production was increased up to 4.8 g/L. The biosurfactant produced by the P. aeruginosa strain was characterized as a mixture of eight different rhamnolipid congeners, being the most abundant the mono-rhamnolipid Rha-C10-C10, and the biosurfactant produced by the B. subtillis isolate consisted of a mixture of C13-, C14- and C15-surfactin. Both biosurfactants exhibited a good performance in oil recovery assays when compared with chemical surfactants, suggesting their potential use as an alternative to traditional chemical surfactants in enhanced oil recovery or bioremediation
