A multi-mycotoxin
method
based
on
High
Performance Liquid Chromatography coupled with tandem
Mass Spectrometry detection
(HPLC-MS
/MS)
was
applied to
investigate the
occurrence
of
toxic
fungal metabolites in
samples
collected
from
a poultry feed factory and integrated poultry
farms,
in
Brazil. A
total
of
119 samples
were analyzed including 38 maize grain samples collected from
the
factory reception, 36 maize grain samples and 9 maize
by-
product samples collected during the factory
processing, and 36 poultry feed samples collected from poultry
farms.
Twenty mycotoxins were
detected
and
quantified in
the
investigated samples: aflatoxins B
1
,
B2,
G
1
,
G2,
fumonisins B
1
,
B2
and
B3,
hydrolyzed fumonisin
B
1
, zearalenone,
agroclavine, chanoclavine, deoxynivalenol, nivalenol,
enniatin A, A
I
,
B,
B,, beauvericin, kojic acid and moniliformin. All samples
were
contaminated with
fumonisins B
1
,
B2
and
B3.
Contamination levels were low (near the LOD) most of toxins, except for
FB
I
and
FB
2
that reach 6,000
mg.mU
l
and 2,450
mg.mL"'
in maize and
1,120
mg.mL"' and
54 mg.mL"
3
in feed,
respectively. This is the
first
study
dealing with agroclavine, chanoclavine, enniatin A, A
1
,
B,
B
1
, beauvericin and
kojic
acid
contamination
of
maize and poultry
feeds
from
Brazil.MYCOTOX - INCO-DEV project (ICA4-CT-2002-10043
