Lima, Nelson

Maciel, M.

Motta, Cristina Souza

Santos, C.

Maciel, Marília

Universidade do Minho: RepositoriUM

124 BIOLOGICAL RESOURCE CENTRES | CLOSING THE GAP BETWEEN SCIENCE AND SOCIETY 125ECCO XXXI MEETING | ABSTRACTS BOOKP62A POLYPHASIC APPROACH TO IDENTIFY CLINICAL ISOLATES OF ASPERGILLUS SECTION NIGRIM. Maciel1,2, C. Santos2, N. Lima2 and C. Souza-Motta11 Centre of Biological Sciences, Department of Mycology, Micoteca URM, Federal University of Pernambuco,  Recife, Brazil2 IBB/Centre of Biological Engineering, Micoteca da Universidade do Minho, Braga, Portugal e-mail: souzamotta@yahoo.com.brAspergillosis is the name of a group of diseases of humans and animals caused by opportunist fungi of the genus Aspergillus. Of the many species of Aspergillus, only few affect humans, the most common being A. fumigatus followed by A. niger. The absence of a reliable fungal identification system is detrimental to the control of systemic fungal infections where specific treatments may be required. Matrix-As-sisted Laser Desorption/Ionisation Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) determines the chemical molecular mass of the microbial cellular composition providing rapid and discriminatory fingerprints for identification and the technique is beginning to be applied in clinical laboratories. This work intended to obtain a reliable identification of Aspergillus isolates from section Nigri deposited at the University of Recife Mycology (URM) culture collection. These materials were used as clinical reference strains to assess the high morphological, biochemical, genomic and proteomic variability of the Brazilian Aspergillus population. A polyphasic ap-proach based on morphological, biochemical and MALDI-TOF MS analyses was applied for the characterisation and identification of 74 Aspergillus isolates from section Nigri deposited at URM. In addition, 12 Aspergillus type strains belonging to section Nigri deposited at Micoteca da Universidade do Minho (MUM) culture col-lection were used as reference strains for MALDI-TOF MS analysis. The polyphasic approach indicated that MALDI-TOF MS results corroborate those obtained using classical taxonomy, biochemistry and molecular biology analyses. MALDI-TOF MS is rapid, reliable and inexpensive in terms of labour and consumables when com-pared with other biological techniques. Finally, the analyses of MALDI-TOF ICMS can be a more rapid and safer alternative for clinical diagnoses. Acknowledgements:Marília Maciel was supported by a grant from Conselho Nacional de Desenvolvimento Científico e Tecnológ-ico (CNPq) Brazil and Erasmus Mundus External Cooperation Window (EMECW) - Lot 17, Portugal.BIOLOGICALRESOURCECENTRESClosing the gapbetween scienceand societyECCO XXXIMEETINGABSTRACTSBOOKEdited byRussell PatersonMarta F. SimõesLeonel PereiraCledir SantosNelson LimaBiological Resource CentresClosing the gap between science and societyEdited byRussell PatersonMarta F. SimõesLeonel PereiraCledir SantosNelson LimaECCO XXXI MeetingAbstracts BookAbstracts of the 31st European Culture Collections’ Organization Meeting, Universidade do Minho, Braga, Portugal, 14-15 June 2012.Biological Resource CentresClosing the gap between science and societyEditors: Russell Paterson,  Marta Simões,  Leonel Pereira,  Cledir Santos,  Nelson LimaPublished by: Micoteca da Universidade do MinhoPrinted: Candeias Artes Gráficas | Braga | PotugalDepósito Legal: 345229/12ISBN: 978-972-97916-5-9Production run: 200 copiesAll rights are reserved to the Micoteca da Universidade do Minho, however the abstracts may be freely reviewed, abstracted, reproduced or translated, in part or in whole, but not for sale or use in conjunction with commercial purposes. The views expressed or implied in this publication, unless otherwise noted, should not be interpreted as official positions of the Micoteca da Universidade do Minho

A polyphasic approach to identify clinical isolates of Aspergillus section Nigri

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